{ "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "FrameKey-0-0-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "ccb6075f-1209-44e1-b41f-548fa36cdbad", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1558393, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:09 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "fb0efa28-51c7-42db-93fc-8e480405f434", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1558908, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "e88f5038-36cf-4101-a62d-6f93c67db1da", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1559427, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "93c1a3a6-4b59-4e83-aadf-a7154cc154c4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1559931, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:11 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "d0aef125-0819-4d34-8654-5938c2fd1c84", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1560447, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:11 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "d19654cc-1140-4001-9b2a-c20cbf551297", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1560955, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:12 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "af707e43-1991-4209-8292-ea2a2826c3d0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1561518, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:12 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "cc122b75-c8bf-4ca1-b732-2ca13a4660d0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1562027, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:13 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "d9db3f1a-6c11-461c-b4a2-5b300f150680", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1562538, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:13 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "7b149865-f560-44d2-a65b-41f56cac7d2c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1563049, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "ca14ab44-2d6c-4567-8b16-1c7f6c34e4ab", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1563567, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "54e31d13-efc4-4d9d-acdc-6198c0a01d65", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1564071, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "3a5d741e-1ec1-4463-a659-87cfa2192ca9", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1564589, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "60d2ca87-ce9e-48e1-9c97-d4c9be54f974", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1565101, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "9b9c0a2e-acc0-4b7b-8c00-ab90bdf3a00e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1565626, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "0f2525e1-6305-46e6-8a68-732aa6b2926d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1566138, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "47dfaa87-2e2e-4330-aa0c-3302a3cd5310", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1566848, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "1f377cea-7180-427c-b367-f7c5e9277cbb", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1568182, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "4ba0d0f4-b9cb-48d6-83b5-c7f249e654b8", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1568496, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "9e591845-96b2-4544-b617-c6deff423fe2", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1568817, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "cf6e58cd-242c-4be3-a65b-f1bac895b879", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1569275, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:20 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "38895cc3-57f4-4486-8dd5-032d8aa7c590", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1569611, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:20 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "871a3dfb-e423-4a55-91bc-21c4e08fb970", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1569930, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b3398f9f-eb12-4e87-b776-804c56890c13", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1570232, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "25345062-c067-42d6-b838-f7dada3e66f0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1570547, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "c838d26b-b0f3-49f3-a4b0-b48742386f1e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1570936, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "33bacb1d-fab5-4782-8d14-95bce6551bae", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1571240, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "289daa8f-6a86-4cef-83d6-0ee6b8331552", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1571548, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "e2e8f9c6-e73f-41b1-9374-247cb180592a", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1571885, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "65241c23-9296-4559-9857-18e6dc5744ed", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1572245, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "d1e6559c-fcd5-41cb-b1a0-f3983005322d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1572550, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "8c79cef7-6151-4a1c-b2e1-adc6c6c71a5a", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1572860, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "e687fc24-87ed-4d48-bce8-c415f1630d45", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1573190, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "fb9ff01a-09e8-4c55-9073-30ffbf5ac95c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1573592, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b31af087-efda-474c-a402-d6a7eb6966ea", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1574350, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:25 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "6edce03c-102b-4608-ab6b-361a0a834773", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1574917, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "9ae1c479-3846-4bf6-8fdd-57ae09932256", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1575285, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "32c669cd-855f-4f2f-8399-06257f871159", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1575637, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "92c4bad3-68b4-4cff-b152-cd39434ec3a0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1576059, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "98047524-325d-4728-9e3b-89d267ed52de", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1576420, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "e9b83e4f-e7ca-468b-a26a-d725e87420ff", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1576771, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "7cf5c0a8-d03c-475a-9f38-8ce3541b2889", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1577137, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:28 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "58afef89-4b2a-472f-a6c7-c5326738b02e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1577513, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:28 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "4b9def0b-ff1a-4973-be47-26e006c02850", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1577881, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:29 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "bb496945-9a5a-4ba0-afd2-2f57c34cdc42", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1578255, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:29 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "bcb97f57-6683-48c9-b00e-3cba0af0ff9e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1578648, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:29 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "637d9f9e-9b96-40eb-8e65-4e9d282b6a5b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1579011, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "3a8e27b7-abd4-4b8b-8e6f-38fe511a85e0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1579380, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "4d56f0ac-910b-4ca8-bf74-c7fe5fb20d59", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1579749, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "14c04109-d539-43f5-820e-0f5d940822ac", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1580152, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:31 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "d37cc1c3-0a9b-442d-95c8-3831df37db47", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1580518, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:31 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b2c59b26-61e4-4223-900a-4e4fac5a2e22", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1581308, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:32 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b8798c37-10eb-4aac-98a5-8881ea3927d4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1581853, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:33 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "07394108-e3e8-48a6-b24a-511a78da5a18", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1582307, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:33 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "bbf2d1e9-0b18-4a19-9787-b941a97eb4cf", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1582771, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:33 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "c4d0737e-b3bc-4634-aaf7-15127db1c73b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1583239, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:34 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "8819fbed-3ce1-4c0c-8d79-78d89d0d4dbc", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1583734, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:34 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "9691bd3f-c968-473d-bc92-0820cfc4cbc2", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1584202, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:35 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "8f996025-0e82-4f19-a370-21bfdf91d1ac", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1584708, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:35 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "a491b994-f783-48df-a98b-740eaa636103", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1585225, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:36 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "1fb66795-8363-404e-9378-1d17c641d88f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1585957, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:37 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "4a4d176c-ee4c-4804-9608-4b1e07e3d617", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1586578, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:37 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "ff597162-1d1b-4ec3-9518-8375bc2e0616", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1587123, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:38 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "fd23eeee-02fb-4676-8149-9a8786312a5d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1587655, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:38 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "31a72af1-21eb-44fd-858a-c54982a88a73", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1588134, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:39 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "8b3abfc7-c2c7-4234-af59-b577b93b870c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1588592, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:39 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "074903ff-3bda-49ef-9180-25e74b0e8bb4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1589068, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "faa8f1f3-0bbb-4fb4-afee-a54f30feb506", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 50, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 16:59:09 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 50, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1589537, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -6520.6, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.015, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 16:59:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5255.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" } }