{ "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "FrameKey-0-0-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "cd9e92da-a807-47c1-900a-4ec37353f59b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1624240, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "d50a376b-0fa3-4f86-850c-3584b3bbab3d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1624752, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "6bc15c9f-1609-42cb-8e4f-d5b71cf69fbb", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1625349, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "3224af1b-4fde-45ab-9f24-130bc2f4002c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1626001, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "54e14ba2-e361-4158-b89c-a6ac84684798", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1626626, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "5da50353-d1ad-4987-b566-c6a71c7427fc", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1627217, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "2db3a021-67ac-448c-a975-b221ba6cee75", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1627743, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "7adb9e4b-435e-43c3-b5a6-dd0f854a0703", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1628266, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "f18144aa-126e-4eab-83b7-1edfc2baabbc", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1628778, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "9671b8b0-94b8-4643-9de4-78eb00d85ac4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1629309, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:20 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "040b6240-da52-450c-ab78-bc67252989eb", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1629821, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:20 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "a30190bc-a166-4fc8-8bb7-d1ee39b20725", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1630328, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "08459d96-8c0b-405c-b580-2ab1ed2f24d7", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1630840, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "df11217c-1dea-4e80-9a43-448f7500d6dd", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1631358, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "be6237e6-f8c5-4ad8-9607-088e42e7851d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1631861, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "a5fff526-b0f3-4667-95fc-6006b8a4214b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1632374, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "1cad8243-fa75-486c-883e-1322536ec244", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1632889, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "74e3fcec-af90-4d4b-a995-f67e1b29965b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1634086, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:25 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "3f44ca40-b8ef-4db4-8446-72c9066f61cb", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1634400, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:25 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "9eefcf7b-66fb-43e6-9d49-e8ad144bb803", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1634714, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:25 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b2976ae3-0b99-42d6-8ec2-f976c2fefcea", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1635017, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "2e617205-a7f9-4205-9444-eb54e95cef70", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1635322, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "c888d187-2177-41e1-a6b0-83c0f6e7cddb", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1635646, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "73157dab-0290-4d32-aa52-07658c679c93", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1635961, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "e5f02140-46b0-4ede-bd6d-3552b554a7d0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1636274, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "1cb88e59-1e3b-4992-801e-3479a7bbba0b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1636606, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "197bc818-5b99-405e-930e-21907bf034ee", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1636919, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:28 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "da134595-ae15-4083-b529-f7817def024d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1637225, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:28 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "cfa968a6-bc5a-4c1c-b8df-11f42d777cde", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1637533, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:28 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "12e8e0a1-e368-4e62-aa6e-2014b5429b45", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1637852, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:29 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "bfffe190-58e8-4e3e-ade8-17f96bd1bd4a", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1638168, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:29 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "a17cc0d9-98a6-4047-a6e3-110b60977d35", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1638471, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:29 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "23796f07-c535-40bc-9944-0a6e91e30160", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1638779, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:29 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "7e50efdf-303d-4900-8db5-c2b64b600178", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1639097, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "14b15a5a-3d32-4e3e-88fd-e492e9ae9d0c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1639791, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b9cf862f-c6f2-4651-bb09-0b44db9131c7", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1640152, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:31 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "30272645-ac91-425a-b34b-5504f657cbde", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1640518, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:31 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "afcdba03-e69d-4222-bda7-d68e0fbd31d8", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1640870, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:32 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "64b49dcd-89e5-402f-ae3e-35d450b49186", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1641239, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:32 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "d2d89d25-1cea-49f5-9b71-bbaa30a486a3", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1641607, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:32 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "49198903-2f85-4c37-8141-c41bfbdfb23d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1641978, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:33 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "2d7af15c-4d00-4c54-a171-d3e92880eea7", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1642341, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:33 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "3cf6de10-a152-4f97-b252-7f84337d4a94", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1642692, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:33 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "08f42d64-4e98-4979-acd3-71d4f01a3e32", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1643044, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:34 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "dc10abe9-31c3-44c3-a76c-0b7bdd222f6b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1643395, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:34 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "c2a08708-bc11-4ded-9592-8c154e6a3c2f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1643747, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:34 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "091f3656-99ab-45b0-9644-9a0d5c83c58e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1644100, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:35 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "103e1b17-2f3c-45c0-85e1-a0605286132d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1644451, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:35 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "a2e6c985-4498-440c-a891-302cb71ba6ce", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1644804, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:35 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "c3e602b4-f8d1-4ae1-81ec-8f6be4d46005", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1645173, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:36 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "c19ec55e-567c-4aa7-9591-cbe76d9e6a4b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1645541, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:36 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "d27a4c97-dc31-43d9-afab-cde72b304835", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1646324, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:37 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "e4421e94-e790-4d39-aadf-a7a73b423100", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1646870, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:38 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "0c001c5a-4d0e-4aff-ba32-42fa5c42791c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1647331, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:38 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "812576e1-b4d2-431e-a8c9-6e718aa718c0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1647796, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:38 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "d1f2564e-80fa-4167-b560-1d7201433c98", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1648258, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:39 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "e56196fa-ba0e-463e-8d66-1454cd0ead90", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1648723, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:39 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "d50960a2-6d2c-4128-9c94-2238e407e3f7", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1649187, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "e1f19690-57ed-4650-a813-12cb604ee048", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1649649, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "a1ff24af-0fc9-46d3-b07e-d8e31d298227", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1650113, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:41 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "a1870511-faba-4d36-85b5-3a27db35a7ff", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1650581, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:41 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "8ca230dd-339d-48f3-98d7-5e5c82ee329e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1651040, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:42 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "00a4a96e-65c4-4674-8363-ff459202a1d3", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1651504, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:42 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "76719de7-2355-4ef3-a533-83e3e2693792", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1651968, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:43 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "189c06c2-be36-4611-a7cd-4a1c5212762b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1652431, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:43 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "ba0a2b1f-ad76-41cc-a500-9247ccfa1069", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1652896, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:44 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "27cedbe8-becc-45dd-b951-f454d26c2a2e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1653357, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:44 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "bee15814-708c-4953-a45f-9afa7a41a5d3", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 52, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 7485.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 7485.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 7485.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8046.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8046.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8046.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38665, 8607.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -38104, 8607.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19999], "PIEZO": [170.03], "XYStage": [ -37543, 8607.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35L_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 8590.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 8590.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -42963.6, 9151.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35L_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19996.7], "PIEZO": [170.02], "XYStage": [ -43524.6, 9151.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35L_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 875.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 875.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 875.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1436.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1436.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1436.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44847.1, 1997.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -44286.1, 1997.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19889.5], "PIEZO": [170.04], "XYStage": [ -43725.1, 1997.5 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 534.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 534.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42173.5, 1095.2 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19870.4], "PIEZO": [170.07], "XYStage": [ -42734.5, 1095.2 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 1491.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 1491.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 1491.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2052.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2052.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2052.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37803.5, 2613.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -37242.5, 2613.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19902.7], "PIEZO": [170.04], "XYStage": [ -36681.5, 2613.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide35R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 2647.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 2647.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42083.2, 3208.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide35R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19917.8], "PIEZO": [170.055], "XYStage": [ -42644.2, 3208.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide35R_Seq02_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -5326.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -5326.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -5326.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4765.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4765.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4765.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -9254.3, -4204.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8693.3, -4204.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19643.7], "PIEZO": [170.015], "XYStage": [ -8132.3, -4204.8 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5816.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5816.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -6520.6, -5255.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19621.4], "PIEZO": [170.015], "XYStage": [ -7081.6, -5255.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -5552.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -5552.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -5552.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4991.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4991.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4991.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1927, -4430.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -1366, -4430.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19575.4], "PIEZO": [170.05], "XYStage": [ -805, -4430.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide25R_Seq02_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3853.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3853.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6386.5, -3292.8 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide25R_Seq02_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19639.2], "PIEZO": [170.005], "XYStage": [ -6947.5, -3292.8 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide25R_Seq02_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "ed08016d-11fa-47d6-bb2e-040ec7e2d025", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-15 17:00:15 -0400", "Date": "2019-06-15", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 52, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq02_2", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 65, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch1" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1653825, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide25R_Seq02_Contra_01", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19998.6000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.3800", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "507.4800", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -1927, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.05, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-15 17:00:44 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -5552.6, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" } }