{
"Summary": {
  "Slices": 17,
  "InitialPositionList": [
    {
      "DeviceCoordinatesUm": {
        "Focus": [2965.9],
        "PIEZO": [169.95],
        "XYStage": [
          -43436.5,
          -5222.2
        ]
      },
      "GridColumnIndex": 0,
      "Label": "EF65ASlide35L_Seq01_Injection_01",
      "GridRowIndex": 0
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2965.9],
        "PIEZO": [169.95],
        "XYStage": [
          -42875.5,
          -5222.2
        ]
      },
      "GridColumnIndex": 1,
      "Label": "EF65ASlide35L_Seq01_Injection_02",
      "GridRowIndex": 0
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2965.9],
        "PIEZO": [169.95],
        "XYStage": [
          -42314.5,
          -5222.2
        ]
      },
      "GridColumnIndex": 2,
      "Label": "EF65ASlide35L_Seq01_Injection_03",
      "GridRowIndex": 0
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2965.9],
        "PIEZO": [169.95],
        "XYStage": [
          -42314.5,
          -4661.2
        ]
      },
      "GridColumnIndex": 2,
      "Label": "EF65ASlide35L_Seq01_Injection_04",
      "GridRowIndex": 1
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2965.9],
        "PIEZO": [169.95],
        "XYStage": [
          -42875.5,
          -4661.2
        ]
      },
      "GridColumnIndex": 1,
      "Label": "EF65ASlide35L_Seq01_Injection_05",
      "GridRowIndex": 1
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2965.9],
        "PIEZO": [169.95],
        "XYStage": [
          -43436.5,
          -4661.2
        ]
      },
      "GridColumnIndex": 0,
      "Label": "EF65ASlide35L_Seq01_Injection_06",
      "GridRowIndex": 1
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2965.9],
        "PIEZO": [169.95],
        "XYStage": [
          -43436.5,
          -4100.2
        ]
      },
      "GridColumnIndex": 0,
      "Label": "EF65ASlide35L_Seq01_Injection_07",
      "GridRowIndex": 2
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2965.9],
        "PIEZO": [169.95],
        "XYStage": [
          -42875.5,
          -4100.2
        ]
      },
      "GridColumnIndex": 1,
      "Label": "EF65ASlide35L_Seq01_Injection_08",
      "GridRowIndex": 2
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2965.9],
        "PIEZO": [169.95],
        "XYStage": [
          -42314.5,
          -4100.2
        ]
      },
      "GridColumnIndex": 2,
      "Label": "EF65ASlide35L_Seq01_Injection_09",
      "GridRowIndex": 2
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2889.2],
        "PIEZO": [170.02],
        "XYStage": [
          -42673.9,
          -11403.4
        ]
      },
      "GridColumnIndex": 0,
      "Label": "EF65ASlide35R_Seq01_Injection_01",
      "GridRowIndex": 0
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2889.2],
        "PIEZO": [170.02],
        "XYStage": [
          -42112.9,
          -11403.4
        ]
      },
      "GridColumnIndex": 1,
      "Label": "EF65ASlide35R_Seq01_Injection_02",
      "GridRowIndex": 0
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2889.2],
        "PIEZO": [170.02],
        "XYStage": [
          -41551.9,
          -11403.4
        ]
      },
      "GridColumnIndex": 2,
      "Label": "EF65ASlide35R_Seq01_Injection_03",
      "GridRowIndex": 0
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2889.2],
        "PIEZO": [170.02],
        "XYStage": [
          -41551.9,
          -10842.4
        ]
      },
      "GridColumnIndex": 2,
      "Label": "EF65ASlide35R_Seq01_Injection_04",
      "GridRowIndex": 1
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2889.2],
        "PIEZO": [170.02],
        "XYStage": [
          -42112.9,
          -10842.4
        ]
      },
      "GridColumnIndex": 1,
      "Label": "EF65ASlide35R_Seq01_Injection_05",
      "GridRowIndex": 1
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2889.2],
        "PIEZO": [170.02],
        "XYStage": [
          -42673.9,
          -10842.4
        ]
      },
      "GridColumnIndex": 0,
      "Label": "EF65ASlide35R_Seq01_Injection_06",
      "GridRowIndex": 1
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2889.2],
        "PIEZO": [170.02],
        "XYStage": [
          -42673.9,
          -10281.4
        ]
      },
      "GridColumnIndex": 0,
      "Label": "EF65ASlide35R_Seq01_Injection_07",
      "GridRowIndex": 2
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2889.2],
        "PIEZO": [170.02],
        "XYStage": [
          -42112.9,
          -10281.4
        ]
      },
      "GridColumnIndex": 1,
      "Label": "EF65ASlide35R_Seq01_Injection_08",
      "GridRowIndex": 2
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2889.2],
        "PIEZO": [170.02],
        "XYStage": [
          -41551.9,
          -10281.4
        ]
      },
      "GridColumnIndex": 2,
      "Label": "EF65ASlide35R_Seq01_Injection_09",
      "GridRowIndex": 2
    }
  ],
  "Interval_ms": 0,
  "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
  "UserName": "Nikon",
  "Depth": 2,
  "PixelType": "GRAY16",
  "Time": "2019-06-14 22:50:08 -0400",
  "Date": "2019-06-14",
  "z-step_um": 3,
  "MetadataVersion": 10,
  "PositionIndex": 14,
  "SlicesFirst": true,
  "ChContrastMin": [
    0,
    0,
    0,
    0
  ],
  "Width": 1200,
  "PixelAspect": 1,
  "MicroManagerVersion": "1.4.23 20180219",
  "ROI": [
    0,
    0,
    1200,
    1200
  ],
  "ChNames": [
    "Il-G",
    "Il-T",
    "Il-A",
    "Il-C"
  ],
  "IJType": 1,
  "GridRow": 0,
  "Comment": "",
  "Height": 1200,
  "GridColumn": 0,
  "Prefix": "Seq01_2",
  "PixelSize_um": 1.1,
  "Frames": 1,
  "CameraTimeout": 20000,
  "BitDepth": 12,
  "KeepShutterOpenChannels": false,
  "Source": "Micro-Manager",
  "Channels": 4,
  "ComputerName": "ZadorConfocal2",
  "CustomIntervals_ms": [],
  "KeepShutterOpenSlices": true,
  "ChColors": [
    -16711681,
    -256,
    -65281,
    -1
  ],
  "TimeFirst": false,
  "ChContrastMax": [
    65536,
    65536,
    65536,
    65536
  ],
  "Positions": 18,
  "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
},
"FrameKey-0-0-0": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -25,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "73f521f4-44b7-42af-a43a-c734ef6c32a2",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 0,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 433755,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_000.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 145.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:08 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-1": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -22,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "c32aa4a9-a0a2-4794-8439-cfdecde78a9b",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 1,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 1,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 434265,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_001.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 148.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:09 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-2": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -19,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "b910cc65-5d89-4c30-9c0e-145991df72ae",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 2,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 2,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 434776,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_002.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 151.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:09 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-3": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -16,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "4a745256-9ec4-47d5-bd9e-e52de56fe22e",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 3,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 3,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 435282,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_003.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 154.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:10 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-4": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -13,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "23f6d26f-faff-4957-b588-ef54d8e09652",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 4,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 4,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 435795,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_004.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 157.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:10 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-5": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -10,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "8d2bc8a7-dad6-460f-982b-a5247a0689e2",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 5,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 5,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 436307,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_005.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 160.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:11 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-6": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -7,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "38ed1823-445d-4dc1-9de4-117d988ff73e",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 6,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 6,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 436819,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_006.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 163.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:11 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-7": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -4,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "e3768cae-98bc-471c-8016-15a5d2524e16",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 7,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 7,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 437331,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_007.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 166.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:12 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-8": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -1,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "31c3df4e-8a41-4363-a2df-c01658537c5b",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 8,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 8,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 437840,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_008.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 169.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:12 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-9": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 2,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "af45da87-29e9-4d55-91b2-594fa4ad6810",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 9,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 9,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 438351,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_009.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 172.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:13 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-10": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 5,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "8f9c98f4-76e9-402f-96ea-b75a2be733ed",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 10,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 10,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 438870,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_010.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 175.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:13 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-11": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 8,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "2e9e8ab8-7f6c-441a-98c0-11fe9a247402",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 11,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 11,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 439376,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_011.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 178.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:14 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-12": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 11,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "214d1d3b-fa25-430b-9efc-4f4a6ec07a6d",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 12,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 12,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 439887,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_012.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 181.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:14 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-13": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 14,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "b08fdd95-75b1-402c-9235-d03378901625",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 13,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 13,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 440402,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_013.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 184.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:15 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-14": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 17,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "194ac68b-c2b6-4d18-93ec-1e1fe089fd29",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 14,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 14,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 440910,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_014.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 187.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:15 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-15": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 20,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "e128eb2d-2c54-4e31-b3a6-7cfee6341174",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 15,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 15,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 441472,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_015.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 190.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:16 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-16": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 23,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "51e110b7-0e04-4c2e-a1ea-cc72c5497592",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 16,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 16,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 441987,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_016.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 193.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:17 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-0": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -25,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "33f0a9cb-6d5b-4a0c-9322-78e3458b3cb8",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 0,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 443189,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_000.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 145.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:18 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-1": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -22,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "2821a285-7f3d-4d72-a5f5-39a1e24f2b6a",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 1,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 1,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 443490,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_001.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 148.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:18 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-2": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -19,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "e7714ceb-e9ed-4ec0-943e-3aff3a3831a5",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 2,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 2,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 443794,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_002.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 151.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:18 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-3": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -16,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "cf942e2e-d726-4743-8401-1a1be50e98e5",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 3,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 3,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 444098,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_003.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 154.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:19 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-4": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -13,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "59f26b04-fa1c-45fc-8b6c-f6e49dde2446",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 4,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 4,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 444402,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_004.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 157.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:19 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-5": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -10,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "eeb9ab75-9e8d-4a98-a6db-99ef94b8fe62",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 5,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 5,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 444705,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_005.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 160.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:19 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-6": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -7,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "a2becf1d-9bb4-4f7c-9934-fd8cadc8ddd6",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 6,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 6,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 445015,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_006.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 163.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:20 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-7": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -4,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "4cea2cca-37ec-43cb-875e-7379c09b43e0",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 7,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 7,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 445330,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_007.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 166.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:20 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-8": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -1,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "b5dede9b-e153-4ba9-96a5-91162a37a4ee",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 8,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 8,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 445634,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_008.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 169.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:20 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-9": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 2,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "895d4b71-bc1e-4c16-b085-45a882515afe",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 9,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 9,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 445937,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_009.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 172.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:21 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-10": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 5,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "73cc4c31-a9fb-4add-bebd-f8f9ad81367a",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 10,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 10,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 446247,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_010.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 175.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:21 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-11": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 8,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "51c5415a-acfe-467c-9bae-d0807863146c",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 11,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 11,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 446562,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_011.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 178.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:21 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-12": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 11,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "480e64c3-61e9-4140-bb4c-1e2c218c4b27",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 12,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 12,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 446864,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_012.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 181.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:21 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-13": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 14,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "2128c523-002e-42da-9023-d1469ffd685c",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 13,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 13,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 447173,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_013.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 184.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:22 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-14": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 17,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "9fa90e9e-d21f-493b-bdc4-796928a30835",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 14,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 14,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 447488,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_014.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 187.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:22 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-15": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 20,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "9d907fbf-c21e-4db8-9f58-5c760d488ab2",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 15,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 15,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 447793,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_015.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 190.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:22 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-16": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 23,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "c7ba4b1e-d9aa-4297-9200-d8ae7431bbde",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 16,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 16,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 448100,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_016.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 193.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:23 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-0": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -25,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "6619149d-557e-473c-826b-3f4a2249bbae",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 0,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 448793,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_000.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 145.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:23 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-1": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -22,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "2f3b86bd-9b53-4822-a34e-3f8093f9f709",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 1,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 1,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 449152,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_001.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 148.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:24 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-2": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -19,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "a1535c83-c33f-4f63-a832-10af1a5b74b2",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 2,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 2,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 449504,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_002.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 151.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:24 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-3": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -16,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "e54b3a73-3de9-4220-99e2-d18b349f821a",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 3,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 3,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 449857,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_003.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 154.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:24 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-4": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -13,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "68ad2a7d-6e56-4c3f-9481-5e7d67d6bd65",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 4,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 4,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 450206,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_004.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 157.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:25 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-5": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -10,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "151c3c94-a6bd-4b55-bae3-71449c3d7819",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 5,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 5,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 450558,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_005.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 160.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:25 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-6": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -7,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "555f235c-17af-4bba-bd6b-a782ef840cdb",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 6,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 6,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 450911,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_006.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 163.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:25 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-7": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -4,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "95ae6e1e-fb45-4097-992f-b2b66a34c4ba",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 7,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 7,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 451262,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_007.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 166.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:26 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-8": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -1,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "b285bfe8-7f72-41e0-ad06-6b265a28ecec",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 8,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 8,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 451613,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_008.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 169.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:26 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-9": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 2,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "a3311473-7c10-4859-b045-2d019a507f7a",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 9,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 9,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 451965,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_009.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 172.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:27 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-10": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 5,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "63037d03-9c4d-4c83-a042-c6ee9da628dd",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 10,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 10,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 452317,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_010.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 175.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:27 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-11": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 8,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "ec106ddb-2233-4df8-bcd6-88a5001d4359",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 11,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 11,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 452673,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_011.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 178.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:27 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-12": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 11,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "7e793fdd-5cbd-4b6e-8a62-0a5a27543b78",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 12,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 12,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 453036,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_012.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 181.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:28 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-13": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 14,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "fb98ac12-8438-46e6-9430-83c110a591d1",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 13,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 13,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 453389,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_013.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 184.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:28 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-14": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 17,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "d7ca996a-9510-4069-8da8-201a23e61e8b",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 14,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 14,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 453741,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_014.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 187.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:28 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-15": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 20,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "a5174723-a832-4b85-bb51-97ea0af2f99a",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 15,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 15,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 454094,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_015.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 190.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:29 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-16": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 23,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "10670325-6d82-43c6-8eaa-a7723c3a3df3",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 16,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 16,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 454448,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_016.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 193.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:29 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-0": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -25,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "ccfafef2-7a91-4d43-a2d1-fc5478d70281",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 0,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 455221,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_000.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 145.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:30 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-1": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -22,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "84c3b66c-be2e-4073-9cbc-4f333154399e",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 1,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 1,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 455679,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_001.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 148.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:30 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-2": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -19,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "4f5a7b22-7387-4a6a-8026-43c612c8efa6",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 2,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 2,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 456143,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_002.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 151.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:31 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-3": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -16,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "d25ba329-b2d4-4229-9d84-ff42e1741535",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 3,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 3,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 456605,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_003.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 154.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:31 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-4": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -13,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "0bb8d48e-891a-4c47-baf0-382fedc8479f",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 4,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 4,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 457069,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_004.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 157.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:32 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-5": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -10,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "3292c08a-0cdd-443a-89b4-c45ca69620fd",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 5,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 5,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 457537,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_005.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 160.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:32 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-6": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -7,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "8563a0d1-4efe-4d55-a8d5-c2143f9cbd45",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 6,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 6,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 457997,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_006.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 163.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:33 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-7": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -4,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "03c8b1a6-313c-4471-ae05-6614b212a737",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 7,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 7,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 458459,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_007.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 166.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:33 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-8": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -1,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "6b34e6e9-2fa8-4452-b4cf-f10225e8345e",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 8,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 8,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 458923,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_008.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 169.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:34 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-9": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 2,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "f8da78c7-27a7-4d52-a770-61a3c3bad552",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 9,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 9,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 459386,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_009.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 172.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:34 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-10": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 5,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "dc251cec-7a3a-43c2-98d1-2e342b4c9e35",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 10,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 10,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 459849,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_010.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 175.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:34 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-11": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 8,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "c93ec0fd-2dfc-4ef9-8ae4-569fea331071",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 11,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 11,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 460315,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_011.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 178.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:35 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-12": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 11,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "1bf2e585-a7ac-4d30-b37f-5933e9f08f2c",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 12,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 12,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 460778,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_012.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 181.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:35 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-13": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 14,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "0ee2dfa3-96cc-4ee3-b86f-2d856a54a4c2",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 13,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 13,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 461247,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_013.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 184.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:36 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-14": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 17,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "bd1da85d-9291-46ed-8c02-9f2a16f416c6",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 14,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 14,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 461704,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_014.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 187.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:36 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-15": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 20,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "2e0b923d-0ffb-4152-9cb0-9445fc7d17ae",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 15,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 15,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 462168,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_015.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 190.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:37 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-16": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 23,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "a2b0fb10-3073-4f5c-9022-70fabb980385",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 14,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:50:08 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 14,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 16,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 16,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 462636,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_06",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -42673.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_016.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 193.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:50:37 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
}
}