{
"Summary": {
  "Slices": 17,
  "InitialPositionList": [
    {
      "DeviceCoordinatesUm": {
        "Focus": [2965.9],
        "PIEZO": [169.95],
        "XYStage": [
          -43436.5,
          -5222.2
        ]
      },
      "GridColumnIndex": 0,
      "Label": "EF65ASlide35L_Seq01_Injection_01",
      "GridRowIndex": 0
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2965.9],
        "PIEZO": [169.95],
        "XYStage": [
          -42875.5,
          -5222.2
        ]
      },
      "GridColumnIndex": 1,
      "Label": "EF65ASlide35L_Seq01_Injection_02",
      "GridRowIndex": 0
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2965.9],
        "PIEZO": [169.95],
        "XYStage": [
          -42314.5,
          -5222.2
        ]
      },
      "GridColumnIndex": 2,
      "Label": "EF65ASlide35L_Seq01_Injection_03",
      "GridRowIndex": 0
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2965.9],
        "PIEZO": [169.95],
        "XYStage": [
          -42314.5,
          -4661.2
        ]
      },
      "GridColumnIndex": 2,
      "Label": "EF65ASlide35L_Seq01_Injection_04",
      "GridRowIndex": 1
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2965.9],
        "PIEZO": [169.95],
        "XYStage": [
          -42875.5,
          -4661.2
        ]
      },
      "GridColumnIndex": 1,
      "Label": "EF65ASlide35L_Seq01_Injection_05",
      "GridRowIndex": 1
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2965.9],
        "PIEZO": [169.95],
        "XYStage": [
          -43436.5,
          -4661.2
        ]
      },
      "GridColumnIndex": 0,
      "Label": "EF65ASlide35L_Seq01_Injection_06",
      "GridRowIndex": 1
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2965.9],
        "PIEZO": [169.95],
        "XYStage": [
          -43436.5,
          -4100.2
        ]
      },
      "GridColumnIndex": 0,
      "Label": "EF65ASlide35L_Seq01_Injection_07",
      "GridRowIndex": 2
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2965.9],
        "PIEZO": [169.95],
        "XYStage": [
          -42875.5,
          -4100.2
        ]
      },
      "GridColumnIndex": 1,
      "Label": "EF65ASlide35L_Seq01_Injection_08",
      "GridRowIndex": 2
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2965.9],
        "PIEZO": [169.95],
        "XYStage": [
          -42314.5,
          -4100.2
        ]
      },
      "GridColumnIndex": 2,
      "Label": "EF65ASlide35L_Seq01_Injection_09",
      "GridRowIndex": 2
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2889.2],
        "PIEZO": [170.02],
        "XYStage": [
          -42673.9,
          -11403.4
        ]
      },
      "GridColumnIndex": 0,
      "Label": "EF65ASlide35R_Seq01_Injection_01",
      "GridRowIndex": 0
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2889.2],
        "PIEZO": [170.02],
        "XYStage": [
          -42112.9,
          -11403.4
        ]
      },
      "GridColumnIndex": 1,
      "Label": "EF65ASlide35R_Seq01_Injection_02",
      "GridRowIndex": 0
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2889.2],
        "PIEZO": [170.02],
        "XYStage": [
          -41551.9,
          -11403.4
        ]
      },
      "GridColumnIndex": 2,
      "Label": "EF65ASlide35R_Seq01_Injection_03",
      "GridRowIndex": 0
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2889.2],
        "PIEZO": [170.02],
        "XYStage": [
          -41551.9,
          -10842.4
        ]
      },
      "GridColumnIndex": 2,
      "Label": "EF65ASlide35R_Seq01_Injection_04",
      "GridRowIndex": 1
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2889.2],
        "PIEZO": [170.02],
        "XYStage": [
          -42112.9,
          -10842.4
        ]
      },
      "GridColumnIndex": 1,
      "Label": "EF65ASlide35R_Seq01_Injection_05",
      "GridRowIndex": 1
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2889.2],
        "PIEZO": [170.02],
        "XYStage": [
          -42673.9,
          -10842.4
        ]
      },
      "GridColumnIndex": 0,
      "Label": "EF65ASlide35R_Seq01_Injection_06",
      "GridRowIndex": 1
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2889.2],
        "PIEZO": [170.02],
        "XYStage": [
          -42673.9,
          -10281.4
        ]
      },
      "GridColumnIndex": 0,
      "Label": "EF65ASlide35R_Seq01_Injection_07",
      "GridRowIndex": 2
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2889.2],
        "PIEZO": [170.02],
        "XYStage": [
          -42112.9,
          -10281.4
        ]
      },
      "GridColumnIndex": 1,
      "Label": "EF65ASlide35R_Seq01_Injection_08",
      "GridRowIndex": 2
    },
    {
      "DeviceCoordinatesUm": {
        "Focus": [2889.2],
        "PIEZO": [170.02],
        "XYStage": [
          -41551.9,
          -10281.4
        ]
      },
      "GridColumnIndex": 2,
      "Label": "EF65ASlide35R_Seq01_Injection_09",
      "GridRowIndex": 2
    }
  ],
  "Interval_ms": 0,
  "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
  "UserName": "Nikon",
  "Depth": 2,
  "PixelType": "GRAY16",
  "Time": "2019-06-14 22:49:07 -0400",
  "Date": "2019-06-14",
  "z-step_um": 3,
  "MetadataVersion": 10,
  "PositionIndex": 12,
  "SlicesFirst": true,
  "ChContrastMin": [
    0,
    0,
    0,
    0
  ],
  "Width": 1200,
  "PixelAspect": 1,
  "MicroManagerVersion": "1.4.23 20180219",
  "ROI": [
    0,
    0,
    1200,
    1200
  ],
  "ChNames": [
    "Il-G",
    "Il-T",
    "Il-A",
    "Il-C"
  ],
  "IJType": 1,
  "GridRow": 0,
  "Comment": "",
  "Height": 1200,
  "GridColumn": 0,
  "Prefix": "Seq01_2",
  "PixelSize_um": 1.1,
  "Frames": 1,
  "CameraTimeout": 20000,
  "BitDepth": 12,
  "KeepShutterOpenChannels": false,
  "Source": "Micro-Manager",
  "Channels": 4,
  "ComputerName": "ZadorConfocal2",
  "CustomIntervals_ms": [],
  "KeepShutterOpenSlices": true,
  "ChColors": [
    -16711681,
    -256,
    -65281,
    -1
  ],
  "TimeFirst": false,
  "ChContrastMax": [
    65536,
    65536,
    65536,
    65536
  ],
  "Positions": 18,
  "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
},
"FrameKey-0-0-0": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -25,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "e738d8a0-1137-400e-8114-76c237a8676d",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 0,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 372701,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_000.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 145.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:07 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-1": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -22,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "762db172-b28c-4da0-b27d-8fb8e1d8041d",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 1,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 1,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 373210,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_001.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 148.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:08 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-2": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -19,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "b8fe3711-536c-49f1-aa1a-6199f29357b6",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 2,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 2,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 373720,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_002.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 151.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:08 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-3": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -16,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "e6aedfc2-7c91-44be-b5e2-a4e733da7f5e",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 3,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 3,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 374236,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_003.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 154.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:09 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-4": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -13,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "2eaa06ec-1fe8-490e-aeb9-b11d4add976a",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 4,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 4,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 374744,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_004.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 157.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:09 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-5": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -10,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "e7201e15-1f91-4cc2-82de-bcc189ced960",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 5,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 5,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 375254,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_005.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 160.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:10 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-6": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -7,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "745bcf50-373c-48f1-82f7-0e9f5a7e7ad2",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 6,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 6,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 375767,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_006.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 163.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:10 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-7": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -4,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "a5d8d925-33ef-4ac9-a82b-175463725c9b",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 7,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 7,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 376279,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_007.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 166.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:11 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-8": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -1,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "eabafb91-516a-4030-85fa-986a2b89b0b6",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 8,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 8,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 376789,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_008.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 169.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:11 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-9": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 2,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "6ea371c4-6f97-4880-90c1-4d0df694d5f3",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 9,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 9,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 377302,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_009.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 172.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:12 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-10": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 5,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "dbd7a701-d1f9-47d4-a4df-3c05543f2540",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 10,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 10,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 377812,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_010.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 175.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:12 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-11": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 8,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "1cfabfd3-360d-4e6e-ae5b-c63f46cabbf4",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 11,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 11,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 378325,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_011.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 178.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:13 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-12": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 11,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "6dc1abb2-8afa-435b-a6e9-dfa3d8dcc86d",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 12,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 12,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 378836,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_012.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 181.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:13 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-13": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 14,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "6bad5a9c-24bd-47c6-bfa6-8b3f03a752bc",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 13,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 13,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 379350,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_013.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 184.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:14 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-14": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 17,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "d579181d-daee-4fe1-bc00-d49262a91e05",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 14,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 14,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 379861,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_014.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 187.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:14 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-15": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 20,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "1",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "a6b258b5-74c9-4423-a8a2-f5f64e1e197c",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 15,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 15,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 380377,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_015.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 190.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:15 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-0-16": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "5.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 23,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 300,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "1f47a774-2d70-42bf-a50f-42f0384f8dfe",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "CFP/YFP",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "565/24(G)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "5.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 16,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "300.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 16,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "520",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 380886,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-G_016.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 193.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-G",
  "XLIGHT Excitation Wheel-Label": "CFP/YFP",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:15 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 0,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-0": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -25,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "288a3578-a9c4-4717-b855-25da2063411f",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 0,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 382082,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_000.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 145.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:17 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-1": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -22,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "6e15e756-4be1-4ae4-9567-6ad9d4f1ce2f",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 1,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 1,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 382398,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_001.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 148.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:17 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-2": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -19,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "8906424b-b5e2-4b30-b9b6-fbdac49c9c30",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 2,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 2,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 382712,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_002.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 151.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:17 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-3": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -16,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "8162d9be-c0ba-415f-acdb-b992944a3fdb",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 3,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 3,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 383016,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_003.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 154.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:18 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-4": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -13,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "0f970b66-2dfb-4825-8185-0872e3133af3",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 4,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 4,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 383319,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_004.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 157.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:18 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-5": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -10,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "2ffcc6c6-1317-452e-88d6-9ee3ce97eab6",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 5,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 5,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 383623,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_005.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 160.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:18 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-6": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -7,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "3eef6a20-236e-43f5-b48a-6c9b08ebf489",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 6,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 6,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 383933,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_006.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 163.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:19 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-7": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -4,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "95187f13-b0a5-4a76-8b0b-fee1d125ae34",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 7,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 7,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 384248,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_007.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 166.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:19 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-8": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -1,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "4054e529-5794-4432-bacd-e238ac11b40f",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 8,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 8,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 384552,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_008.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 169.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:19 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-9": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 2,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "44d1f502-defc-4d04-9b16-d6bb96278474",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 9,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 9,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 384855,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_009.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 172.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:19 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-10": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 5,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "12035403-50fc-4fbd-8109-53a3646c330e",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 10,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 10,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 385158,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_010.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 175.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:20 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-11": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 8,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "e71cc211-4059-493a-bd45-44ed9e6318b8",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 11,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 11,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 385462,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_011.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 178.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:20 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-12": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 11,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "023538c7-44b2-496a-bc75-6a9202dad275",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 12,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 12,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 385766,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_012.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 181.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:20 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-13": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 14,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "f7cc3ef6-8a5a-4c56-8027-6caec8a04c76",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 13,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 13,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 386070,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_013.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 184.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:21 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-14": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 17,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "a6ffd303-e198-4d50-8078-8ba73d941c2a",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 14,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 14,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 386374,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_014.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 187.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:21 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-15": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 20,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "2507ae46-a2f9-4bb4-97c0-2df5892e9bb5",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 15,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 15,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 386679,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "1",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_015.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 190.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:21 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-1-16": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 23,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 100,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "b3b9e2d9-b9a6-4942-9629-c26349ecc464",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "585/11(T)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 16,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "100.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 16,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "555",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 386990,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "0.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-T_016.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 193.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-T",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:22 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 1,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "0.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "5.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "5.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-0": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -25,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "70f635a0-67ab-4787-9e7d-7267b0fce415",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 0,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 387684,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_000.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 145.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:22 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-1": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -22,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "e5b3b260-ab75-46f8-acaa-4a9a6aaf675e",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 1,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 1,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 388059,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_001.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 148.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:23 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-2": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -19,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "c8193a97-f09e-4f87-a662-e30d130fd7c3",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 2,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 2,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 388421,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_002.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 151.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:23 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-3": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -16,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "7d71abe7-a510-40ac-903e-212e948bc7c4",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 3,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 3,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 388773,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_003.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 154.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:23 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-4": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -13,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "5bbed09b-c966-4487-bcd5-607db46d841e",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 4,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 4,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 389124,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_004.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 157.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:24 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-5": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -10,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "fb1ac91f-e5ec-4ac9-bc96-8984dba44f6f",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 5,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 5,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 389476,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_005.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 160.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:24 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-6": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -7,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "4408e871-118b-414e-9ebf-bb3db63f4834",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 6,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 6,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 389833,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_006.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 163.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:24 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-7": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -4,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "4fa1f812-b8e7-479c-9147-1269de2450ff",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 7,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 7,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 390196,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_007.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 166.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:25 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-8": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -1,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "3b281e26-5eb9-422d-a461-77546c20d1ab",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 8,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 8,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 390548,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_008.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 169.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:25 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-9": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 2,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "c6a5870e-859b-48b5-b4f3-91e795bd57a3",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 9,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 9,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 390903,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_009.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 172.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:25 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-10": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 5,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "ec78440f-078a-4237-b5b7-9689f8c1fecd",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 10,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 10,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 391268,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_010.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 175.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:26 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-11": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 8,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "17564174-1782-4dbe-ab2d-e1a71f33c6cf",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 11,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 11,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 391620,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_011.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 178.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:26 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-12": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 11,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "6d5157d2-63f9-41ec-9852-62d57fbdeb42",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 12,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 12,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 391975,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_012.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 181.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:27 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-13": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 14,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "38ea922e-a6c0-4c63-a0e7-e21257c4bdf7",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 13,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 13,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 392340,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_013.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 184.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:27 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-14": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 17,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "5a28406a-9851-4a0e-a36d-c567125947d4",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 14,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 14,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 392691,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_014.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 187.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:27 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-15": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 20,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "a7f4d30b-2e1a-4683-8998-f043fcdd4782",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 15,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 15,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 393063,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_015.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 190.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:28 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-2-16": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 23,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 150,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "215c97eb-9537-4ca1-bb58-8c2adfdba937",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "679/29 (A)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 16,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "150.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 16,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 393429,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-A_016.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 193.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-A",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:28 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 2,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-0": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -25,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "6180e27f-d32f-4ef3-b9c0-5cbf1e1811b0",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 0,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 394204,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_000.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 145.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:29 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-1": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -22,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "bc8bb281-2cd9-45b2-a829-109c8e089ef5",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 1,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 1,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 394660,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_001.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 148.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:29 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-2": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -19,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "d6aaa649-0389-49d3-88fd-f53ccbdf8456",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 2,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 2,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 395123,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_002.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 151.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:30 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-3": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -16,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "ec6207aa-eb31-41e9-ad05-ede396104860",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 3,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 3,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 395588,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_003.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 154.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:30 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-4": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -13,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "1870d98d-95eb-4055-96b5-7693f9275a18",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 4,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 4,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 396052,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_004.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 157.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:31 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-5": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -10,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "c1f520d3-194d-4bd8-bb93-ddb579635907",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 5,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 5,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 396515,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_005.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 160.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:31 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-6": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -7,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "c1124660-17de-4960-969b-9c01871e71a3",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 6,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 6,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 396978,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_006.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 163.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:32 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-7": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -4,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "f9fc4f66-e154-4ab4-b4c7-1d6611725a20",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 7,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 7,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 397446,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_007.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 166.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:32 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-8": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": -1,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "91f8af8d-7fd2-408b-8d3c-805244e80f75",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 8,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 8,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 397908,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_008.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 169.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:32 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-9": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 2,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "94c54b3e-da8a-4815-9989-f145d640634d",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 9,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 9,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 398370,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_009.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 172.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:33 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-10": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 5,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "47688c0a-c101-423a-b903-ad1d4d67d4c7",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 10,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 10,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 398838,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_010.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 175.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:33 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-11": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 8,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "3fd5b3a0-5e6e-478f-a8f5-ba35f6bc9a76",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 11,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 11,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 399296,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_011.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 178.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:34 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-12": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 11,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "6e6fe25a-5aa0-4abd-a423-15d88a19a44a",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 12,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 12,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 399760,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_012.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 181.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:34 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-13": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 14,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "bbc67514-ef1b-429d-bb6d-0be8f056fc85",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 13,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 13,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 400224,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_013.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 184.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:35 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-14": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 17,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "564488d7-db4b-43dd-ad05-18a9b1e9d4bd",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 14,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 14,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 400687,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_014.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 187.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:35 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-15": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 20,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "a8e409d2-8345-4afd-a776-3e6552377709",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 15,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 15,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 401151,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_015.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 190.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:36 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "1",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
},
"FrameKey-0-3-16": {
  "Core-Initialize": "1",
  "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
  "405-DA Device": "405 Shutter",
  "PRIME-Y-dimension": "1200",
  "470 Shutter-Block voltage": "false",
  "PRIME-MetadataEnabled": "No",
  "555-Name": "DA Shutter",
  "PRIME-TransposeMirrorX": "0",
  "PRIME-TransposeMirrorY": "0",
  "640 AO-Demo": "No",
  "405 Shutter-MaxVolts": "5.0000",
  "COM1-Description": "Serial port driver (boost:asio)",
  "470-Name": "DA Shutter",
  "520 AO-Volts": "0.0000",
  "ROI": "0-0-1200-1200",
  "520 AO-Description": "NI DAC",
  "Vincent-D3-Address": "x",
  "PRIME-PP  5   ENABLED": "No",
  "555 Shutter-MaxVolts": "5.0000",
  "470 AO-Description": "NI DAC",
  "470-State": "0",
  "PRIME-Offset": "100",
  "SlicePosition": 23,
  "640 Shutter-TriggerSampleRate": "100000",
  "Exposure-ms": 250,
  "405 AO-MaxVolts": "5.0000",
  "640-Description": "DA device that is used as a shutter",
  "445 Shutter-Volts": "0.0000",
  "XLIGHT Disk slider-Description": "XLIGHT Disk slider",
  "640 AO-Sequenceable": "1",
  "405-Description": "DA device that is used as a shutter",
  "445-DA Device": "445 Shutter",
  "470 AO-MinVolts": "0.0000",
  "PRIME-Timing-PostTriggerDelayNs": "24672000.0000",
  "555 Shutter-MinVolts": "0.0000",
  "405 AO-MinVolts": "0.0000",
  "AxisPositions": null,
  "PRIME-PP  4   ENABLED": "Yes",
  "PRIME-CCDTemperatureSetPoint": "-15.0000",
  "COM9-DelayBetweenCharsMs": "0.0000",
  "PRIME-ChipName": "GS144BSI",
  "COM1-Parity": "None",
  "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
  "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
  "520 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM9-BaudRate": "9600",
  "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
  "Focus-Name": "Focus",
  "COM1-StopBits": "1",
  "445 Shutter-IOChannel": "Dev1/ao9",
  "PIEZO-LudlSingleAxisName": "B",
  "PRIME-PP  1   ENABLED": "Yes",
  "640 Shutter-IOChannel": "Dev1/ao14",
  "520 AO-TriggerSequenceLength": "1000",
  "528 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "PRIME-PP  2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
  "FilterBlock-State": "5",
  "445-Description": "DA device that is used as a shutter",
  "445-Name": "DA Shutter",
  "COM3-Handshaking": "Off",
  "405 AO-Description": "NI DAC",
  "520-State": "0",
  "PRIME-FullWellCapacity": "62000",
  "UUID": "031e60ed-bb33-40be-a3a9-94444f49f0a4",
  "PRIME-ClearMode": "Pre-Exposure",
  "Core-Camera": "PRIME",
  "Core-SLM": "",
  "528 AO-Block voltage": "false",
  "470 Shutter-MinVolts": "0.0000",
  "XLIGHT Disk slider-State": "2",
  "TE2000-Name": "TE2000",
  "COM1-Handshaking": "Off",
  "Core-ChannelGroup": "Channels",
  "PositionIndex": 12,
  "WaitInterval": null,
  "XLIGHT Disk slider-Label": "Disk Pos 40um",
  "555-DA Device": "555 Shutter",
  "470 AO-MaxVolts": "5.0000",
  "445 Shutter-Demo": "No",
  "445 AO-TriggerSampleRate": "100000",
  "528 AO-Description": "NI DAC",
  "470 Shutter-SupportsTriggering": "0",
  "528 AO-MinVolts": "0.0000",
  "528 Shutter-Sequenceable": "1",
  "470 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  3   THRESHOLD": "80",
  "520 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  6 Reset": "No",
  "PRIME-PP  3   ENABLED": "Yes",
  "Summary": {
    "Slices": 17,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -5222.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4661.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -43436.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35L_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42875.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35L_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2965.9],
          "PIEZO": [169.95],
          "XYStage": [
            -42314.5,
            -4100.2
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35L_Seq01_Injection_09",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_01",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_02",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -11403.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_03",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_04",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_05",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10842.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_06",
        "GridRowIndex": 1
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42673.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 0,
        "Label": "EF65ASlide35R_Seq01_Injection_07",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -42112.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 1,
        "Label": "EF65ASlide35R_Seq01_Injection_08",
        "GridRowIndex": 2
      },
      {
        "DeviceCoordinatesUm": {
          "Focus": [2889.2],
          "PIEZO": [170.02],
          "XYStage": [
            -41551.9,
            -10281.4
          ]
        },
        "GridColumnIndex": 2,
        "Label": "EF65ASlide35R_Seq01_Injection_09",
        "GridRowIndex": 2
      }
    ],
    "Interval_ms": 0,
    "UUID": "39249a8d-160a-47dc-a991-69fb2bd2aede",
    "UserName": "Nikon",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2019-06-14 22:49:07 -0400",
    "Date": "2019-06-14",
    "z-step_um": 3,
    "MetadataVersion": 10,
    "PositionIndex": 12,
    "SlicesFirst": true,
    "ChContrastMin": [
      0,
      0,
      0,
      0
    ],
    "Width": 1200,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.23 20180219",
    "ROI": [
      0,
      0,
      1200,
      1200
    ],
    "ChNames": [
      "Il-G",
      "Il-T",
      "Il-A",
      "Il-C"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1200,
    "GridColumn": 0,
    "Prefix": "Seq01_2",
    "PixelSize_um": 1.1,
    "Frames": 1,
    "CameraTimeout": 20000,
    "BitDepth": 12,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 4,
    "ComputerName": "ZadorConfocal2",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": true,
    "ChColors": [
      -16711681,
      -256,
      -65281,
      -1
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536,
      65536
    ],
    "Positions": 18,
    "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A"
  },
  "Source": "PRIME",
  "PRIME-Name": "PMPCIECam00",
  "COM9-Name": "COM9",
  "XLIGHT Dichroic-Label": "QUAD",
  "COM4-StopBits": "1",
  "555 Shutter-TriggerSampleRate": "100000",
  "445 AO-MaxVolts": "5.0000",
  "PRIME-PP  1   THRESHOLD": "125",
  "405 AO-AutoDetectedOutputPort": "Dev1/ao0",
  "640 Shutter-Name": "AnalogIO",
  "555 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-MaxVolts": "5.0000",
  "PRIME-PMode": "Normal",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "TE2000-Port": "COM4",
  "520 Shutter-MaxVolts": "5.0000",
  "470 Shutter-MaxVolts": "5.0000",
  "PRIME-TransposeXY": "0",
  "640 AO-IOChannel": "Dev1/ao7",
  "555 AO-TriggerSequenceLength": "1000",
  "XLIGHT Emission Wheel-Label": "725/40 (C)",
  "COM3-Parity": "None",
  "405-State": "0",
  "520 Shutter-Volts": "0.0000",
  "405-Name": "DA Shutter",
  "PRIME-TransposeCorrection": "0",
  "XLIGHT Dichroic-Name": "XLIGHT Dichroic",
  "Vincent-D3-Name": "Vincent-D3",
  "PRIME-PreampOffLimit": "10000",
  "PRIME-PP  4   MIN ADU AFFECTED": "200",
  "LudlController-Version": "Version no. :      13.500   MAC6000 Interface with USB/ETH  ",
  "Core-Galvo": "",
  "Position": "Default",
  "COM9-Handshaking": "Off",
  "CameraChannelIndex": 0,
  "SliceIndex": 16,
  "Core-Focus": "PIEZO",
  "COM1-Verbose": "1",
  "445 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-SupportsTriggering": "0",
  "FrameIndex": 0,
  "PRIME-Exposure": "250.00",
  "405 AO-Demo": "No",
  "PRIME-PP  4   THRESHOLD": "75",
  "PRIME-CircularBufferAutoSize": "ON",
  "520 AO-TriggerSampleRate": "100000",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "520 AO-AutoDetectedOutputPort": "Dev1/ao4",
  "XLIGHT Spin motor-Name": "XLIGHT Spin motor",
  "PRIME-Description": "PVCAM API device adapter",
  "405 AO-Sequenceable": "1",
  "528 Shutter-Volts": "0.0000",
  "TE2000-ModelType": "1",
  "445 Shutter-Block voltage": "false",
  "Core-AutoShutter": "1",
  "PRIME-AcquisitionMethod": "Callbacks",
  "PRIME-ShutterMode": "Pre-Exposure",
  "445 Shutter-SupportsTriggering": "0",
  "Core-XYStage": "XYStage",
  "PRIME-PP  2   ENABLED": "Yes",
  "XYStage-StepSize-X": "0.1000",
  "XYStage-StepSize-Y": "0.1000",
  "PRIME-Color": "OFF",
  "COM9-Description": "Serial port driver (boost:asio)",
  "528 AO-Sequenceable": "1",
  "528 Shutter-Name": "AnalogIO",
  "405 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-AutoDetectedOutputPort": "Dev1/ao7",
  "405 Shutter-Description": "NI DAC",
  "405 Shutter-IOChannel": "Dev1/ao8",
  "Core-AutoFocus": "",
  "PRIME-Timing-ExposureTimeNs": "100003000.0000",
  "470 Shutter-Volts": "0.0000",
  "640 Shutter-Description": "NI DAC",
  "PIEZO-Description": "Ludl stage driver adapter",
  "640 AO-MinVolts": "0.0000",
  "BitDepth": 12,
  "Slice": 16,
  "LudlController-Description": "Ludl Controller (Mac 2000/5000)",
  "470 AO-TriggerInputLine": "/Dev1/PFI0",
  "COM4-Verbose": "1",
  "555 Shutter-SupportsTriggering": "0",
  "Core-Shutter": "640",
  "528 AO-TriggerSequenceLength": "1000",
  "PRIME-PP  1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
  "XLIGHT Hub-Port": "COM3",
  "TE2000-Version": "V2.50-V2.50-V2.10",
  "520 AO-MaxVolts": "5.0000",
  "520 Shutter-Description": "NI DAC",
  "ElapsedTime-ms": 401618,
  "640 Shutter-Sequenceable": "1",
  "470 Shutter-Description": "NI DAC",
  "445-State": "0",
  "Frame": 0,
  "520-Name": "DA Shutter",
  "PRIME-Trigger-Expose Out-Mux": "1",
  "OpticalPath-State": "4",
  "PositionName": "EF65ASlide35R_Seq01_Injection_04",
  "XLIGHT Spin motor-Label": "On (spinning)",
  "640 AO-TriggerSampleRate": "100000",
  "COM9-AnswerTimeout": "10000.0000",
  "520 Shutter-SupportsTriggering": "0",
  "Height": 1200,
  "555 AO-TriggerInputLine": "/Dev1/PFI0",
  "470 Shutter-TriggerSampleRate": "100000",
  "555 AO-Demo": "No",
  "LudlController-TransmissionDelay": "0",
  "PRIME-Port": "CMOS",
  "445 Shutter-Name": "AnalogIO",
  "COM3-Name": "COM3",
  "PRIME-SMARTStreamingEnabled": "No",
  "445 Shutter-TriggerSampleRate": "100000",
  "LudlController-Reset": "Operate",
  "Vincent-D3-Description": "Vincent D3 controller adapter",
  "520 Shutter-Name": "AnalogIO",
  "PRIME-CircularBufferEnabled": "ON",
  "470 AO-Name": "AnalogIO",
  "PRIME-Timing-ReadoutTimeNs": "12336000.0000",
  "640 Shutter-MinVolts": "0.0000",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "640 Shutter-TriggerSequenceLength": "1000",
  "PRIME-PP  2   THRESHOLD": "125",
  "528 AO-TriggerInputLine": "/Dev1/PFI0",
  "640 Shutter-SupportsTriggering": "0",
  "520 AO-Demo": "No",
  "PRIME-FTCapable": "No",
  "470 AO-AutoDetectedOutputPort": "Dev1/ao2",
  "445 AO-IOChannel": "Dev1/ao1",
  "Vincent-D3-Time to open (ms)": "35.0000",
  "PRIME-PP  4 QUANTVIEW": "QUANTVIEW",
  "XLIGHT Disk slider-Name": "XLIGHT Disk slider",
  "405 Shutter-Sequenceable": "1",
  "445 Shutter-MinVolts": "0.0000",
  "528 AO-Demo": "No",
  "445 Shutter-TriggerSequenceLength": "1000",
  "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
  "528 Shutter-TriggerSampleRate": "100000",
  "405 AO-Volts": "0.0000",
  "555 AO-IOChannel": "Dev1/ao6",
  "PRIME-ShutterOpenDelay": "0",
  "528-State": "0",
  "640-Name": "DA Shutter",
  "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
  "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
  "405 Shutter-TriggerSequenceLength": "1000",
  "Focus-Position": "2959.7000",
  "COM1-AnswerTimeout": "500.0000",
  "640 Shutter-Block voltage": "false",
  "528 Shutter-IOChannel": "Dev1/ao12",
  "555 Shutter-Demo": "No",
  "640 AO-Name": "AnalogIO",
  "PRIME-Binning": "1x1",
  "470 AO-Sequenceable": "1",
  "PRIME-PP  0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
  "520 Shutter-MinVolts": "0.0000",
  "470 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "640 AO-TriggerSequenceLength": "1000",
  "520 AO-Sequenceable": "1",
  "XYStage-Name": "Stage",
  "405 Shutter-Block voltage": "false",
  "520 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555 AO-TriggerSampleRate": "100000",
  "555 Shutter-Sequenceable": "1",
  "PRIME-Trigger Timeout (secs)": "10",
  "640 Shutter-Volts": "5.0000",
  "LudlController-Name": "LudlController",
  "555 Shutter-Name": "AnalogIO",
  "XLIGHT Spin motor-State": "1",
  "445 AO-Demo": "No",
  "PRIME-CCDTemperature": "-14.9900",
  "470 AO-IOChannel": "Dev1/ao2",
  "Focus-Description": "Nikon TE2000 focus adapter",
  "555-State": "0",
  "COM3-AnswerTimeout": "500.0000",
  "520 AO-Name": "AnalogIO",
  "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
  "555 AO-SupportsTriggering": "0",
  "XYStage-StartSpeed": "500.0000",
  "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
  "520 Shutter-Block voltage": "false",
  "640-DA Device": "640 Shutter",
  "PRIME-PVCAM Version": "3.7.1",
  "640 AO-SupportsTriggering": "0",
  "Vincent-D3-Channel #": "Ch. #1",
  "470-Description": "DA device that is used as a shutter",
  "XLIGHT Emission Wheel-State": "5",
  "XYStage-Speed": "1800.0000",
  "528 AO-TriggerSampleRate": "100000",
  "405 Shutter-Demo": "No",
  "Focus-FocusStepSize_(nm)": "100",
  "528 AO-AutoDetectedOutputPort": "Dev1/ao5",
  "520 AO-MinVolts": "0.0000",
  "PixelSizeUm": 1.1,
  "528 AO-MaxVolts": "5.0000",
  "445 Shutter-MaxVolts": "5.0000",
  "640 AO-Block voltage": "false",
  "555 AO-Name": "AnalogIO",
  "PRIME-ActualInterval-ms": "165.2300",
  "COM9-StopBits": "2",
  "528 Shutter-TriggerSequenceLength": "1000",
  "COM4-DataBits": "8",
  "520 Shutter-Sequenceable": "1",
  "405 Shutter-Volts": "0.0000",
  "COM3-DataBits": "8",
  "470 AO-Demo": "No",
  "555 AO-Sequenceable": "1",
  "PRIME-ExposeOutMode": "First Row",
  "Binning": "1x1",
  "405 Shutter-SupportsTriggering": "0",
  "555 AO-Block voltage": "false",
  "528 AO-SupportsTriggering": "0",
  "520 Shutter-TriggerSampleRate": "100000",
  "PRIME-PP  3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
  "Vincent-D3-Command": "Close",
  "COM9-Verbose": "1",
  "470 Shutter-Name": "AnalogIO",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "520 Shutter-IOChannel": "Dev1/ao11",
  "PRIME-SerialNumber": "A18B203008",
  "PRIME-PixelType": "12bit",
  "405 AO-Block voltage": "false",
  "405 Shutter-Name": "AnalogIO",
  "XYStage-TransposeMirrorY": "0",
  "445 AO-AutoDetectedOutputPort": "Dev1/ao1",
  "XPositionUm": -41551.9,
  "XYStage-TransposeMirrorX": "0",
  "470-DA Device": "470 Shutter",
  "640 AO-TriggerInputLine": "/Dev1/PFI0",
  "528 Shutter-Block voltage": "false",
  "FileName": "img_000000000_Il-C_016.tif",
  "528 Shutter-Description": "NI DAC",
  "XYStage-Acceleration": "4.0000",
  "555 Shutter-Description": "NI DAC",
  "PRIME-TriggerMode": "Internal Trigger",
  "445 AO-Description": "NI DAC",
  "470 AO-Volts": "0.0000",
  "ZPositionUm": 193.02,
  "528-DA Device": "528 Shutter",
  "640 AO-Description": "NI DAC",
  "Channel": "Il-C",
  "XLIGHT Excitation Wheel-Label": "QUAD",
  "470 Shutter-Sequenceable": "1",
  "528 Shutter-Demo": "No",
  "405 Shutter-MinVolts": "0.0000",
  "TE2000-Description": "Nikon TE2000 hub",
  "FilterBlock-Label": "Confocal",
  "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
  "PRIME-FirmwareVersion": "20.28",
  "XYStage-StepSize": "0.1000",
  "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
  "640 Shutter-MaxVolts": "5.0000",
  "555 AO-MinVolts": "0.0000",
  "640 Shutter-Demo": "No",
  "PRIME-Gain": "1-Full well",
  "555 Shutter-TriggerSequenceLength": "1000",
  "PRIME-Timing-PreTriggerDelayNs": "12336000.0000",
  "Camera": "",
  "445 AO-Sequenceable": "1",
  "PRIME-PP  2   MIN ADU AFFECTED": "200",
  "520 AO-SupportsTriggering": "0",
  "XLIGHT Hub-Name": "XLIGHT Hub",
  "PRIME-Timing-ClearingTimeNs": "24672000.0000",
  "PRIME-PreampDelay": "0",
  "445 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "COM1-DataBits": "8",
  "520 AO-IOChannel": "Dev1/ao4",
  "470 Shutter-Demo": "No",
  "555 AO-MaxVolts": "5.0000",
  "520-DA Device": "520 Shutter",
  "405 AO-Name": "AnalogIO",
  "405 AO-TriggerSampleRate": "100000",
  "XLIGHT Dichroic-State": "0",
  "NextFrame": 0,
  "COM9-Parity": "None",
  "528-Description": "DA device that is used as a shutter",
  "445 Shutter-Sequenceable": "1",
  "555 AO-AutoDetectedOutputPort": "Dev1/ao6",
  "Time": "2019-06-14 22:49:36 -0400",
  "405 AO-IOChannel": "Dev1/ao0",
  "PRIME-FanSpeedSetpoint": "High",
  "COM4-Name": "COM4",
  "PRIME-ReadoutRate": "200MHz 12bit",
  "COM4-BaudRate": "9600",
  "445 Shutter-Description": "NI DAC",
  "640-State": "0",
  "470 AO-Block voltage": "false",
  "528 AO-Volts": "0.0000",
  "640 AO-MaxVolts": "5.0000",
  "445 AO-Block voltage": "false",
  "520-Description": "DA device that is used as a shutter",
  "OpticalPath-Description": "Nikon TE2000 optical path adapter",
  "PRIME-ShutterCloseDelay": "0",
  "PRIME-CircularBufferFrameCount": "46",
  "555 Shutter-Block voltage": "false",
  "PIEZO-StepSize": "0.0050",
  "445 AO-TriggerSequenceLength": "1000",
  "COM9-DataBits": "8",
  "520 AO-Block voltage": "false",
  "405 AO-TriggerInputLine": "/Dev1/PFI0",
  "ChannelIndex": 3,
  "445 AO-MinVolts": "0.0000",
  "PRIME-ClearCycles": "2",
  "PRIME-X-dimension": "1200",
  "Core-TimeoutMs": "5000",
  "FilterBlock-Name": "FilterBlock",
  "PRIME-CircularBufferFrameRecovery": "OFF",
  "470 Shutter-TriggerSequenceLength": "1000",
  "640 Shutter-TriggerInputLine": "/Dev1/PFI0",
  "555-Description": "DA device that is used as a shutter",
  "OpticalPath-Name": "OpticalPath",
  "Vincent-D3-Time to close (ms)": "35.0000",
  "Width": 1200,
  "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
  "445 AO-SupportsTriggering": "0",
  "470 AO-SupportsTriggering": "0",
  "COM3-StopBits": "1",
  "PIEZO-Name": "Stage",
  "528 Shutter-MinVolts": "0.0000",
  "COM3-Verbose": "1",
  "640 AO-Volts": "5.0000",
  "528-Name": "DA Shutter",
  "470 Shutter-IOChannel": "Dev1/ao10",
  "Vincent-D3-Port": "COM1",
  "520 Shutter-Demo": "No",
  "XLIGHT Hub-Description": "XLIGHT hub",
  "555 Shutter-IOChannel": "Dev1/ao13",
  "XYStage-Description": "Ludl XY stage driver adapter",
  "555 AO-Description": "NI DAC",
  "COM4-Handshaking": "Off",
  "XLIGHT Excitation Wheel-State": "0",
  "405 AO-SupportsTriggering": "1",
  "528 AO-Name": "AnalogIO",
  "405 AO-TriggerSequenceLength": "1000",
  "LudlController-Port": "COM9",
  "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
  "555 AO-Volts": "0.0000",
  "405 Shutter-TriggerSampleRate": "100000",
  "COM4-AnswerTimeout": "500.0000",
  "OpticalPath-Label": "Left(100)",
  "YPositionUm": -10842.4,
  "COM1-BaudRate": "9600",
  "445 AO-Name": "AnalogIO",
  "555 Shutter-Volts": "0.0000",
  "528 AO-IOChannel": "Dev1/ao5",
  "445 AO-Volts": "0.0000",
  "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
  "470 AO-TriggerSampleRate": "100000",
  "COM3-BaudRate": "9600",
  "COM1-Name": "COM1",
  "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
  "PRIME-PVCAM Adapter Version": "1.3.29"
}
}