DateAndTime=2022-03-02 14:45:11 NumberOfTimePoints=1 NumberOfZPoints=230 NumberOfChannels=2 Width=2048 Height=2048 FusionVersion=2.3.0.36 Protocol Name=ZScanProtocolWizard [Protocol Specification] [TimeSeriesProtocolSpecification] IsOuterRepeat=False RepeatCount=1 Duration=74.576 ms IsFixedDuration=False ActualDuration=74.576 ms Interval=0 ms ActualInterval=74576 µs RepeatsPerSecond=0 RepeatsMode=Repeats IntervalMode=Fastest IsValid=True IsActive=True [TimeSeriesProtocolSpecification] IsOuterRepeat=True RepeatCount=1 Duration=74.576 ms IsFixedDuration=False ActualDuration=74.576 ms Interval=0 ms ActualInterval=74576 µs RepeatsPerSecond=0 RepeatsMode=Repeats IntervalMode=Interval IsValid=True IsActive=False Name=TimeSeriesOuterRepeat [TimeOptionsProtocolSpecification] NumberOfNestedRepeats=0 IsValid=True [ZScanProtocolSpecification] Scale=Full BoundedZMinimum=-500000 BoundedZMaximum=500000 IsNegativeScan=False ZScanProtocolOrder=RunZScansAtEachChannel UseRefZForScans=False ActualStartPosition=133.9 ActualEndPosition=591.9 StartPosition=133.9 EndPosition=592.4 CentrePosition=363.15 Size=458.5 StepSize=2 StepCount=230 StepSizeMode=StepSizeDefinition ScanDefinitionMode=StartEnd IsProtocolElementActive=True IsValid=True [MontageProtocolSpecification] IsMontageAvailable=True IsViewExpanded=True IsMontageEnabled=True CurrentMontageMode=Edge IsValid=True [EdgeMontageProtocolSpecification] Overlap=5 IsValid=True [MultiFieldDefinitions] [FieldDefinition] X=34.013900 Y=2.973200 FieldName=1 IsValid=True [FieldDefinition] X=38.109800 Y=4.551800 FieldName=2 IsValid=True [FieldDefinition] X=39.110200 Y=-1.841500 FieldName=3 IsValid=True [FieldDefinition] X=36.788300 Y=-1.960300 FieldName=4 IsValid=True Rows=7 Columns=6 [FieldMontageProtocolSpecification] Overlap=10 IsValid=True Rows=3 Columns=10 CurrentPosition=TopLeft [ChannelProtocolSpecification] [ChannelGroups] [ChannelGroup] [Channel] Wavelength=521 nm IsPrimaryChannel=True AutoGenerateColour=False EIRMode=Full AcquisitionDeviceAlias=Zyla 2 ImagingMode={Name=CF40} Name=488_GFP_CF40_Zyla 2 Description= IsActive=True IsReadOnly=False [FeatureValueList] {DisplayName=Rotation, Value=0˚} {DisplayName=Rotation, Value=0˚} {DisplayName=Flip Vertical, Value=True} {DisplayName=Flip Vertical, Value=True} {DisplayName=Flip Horizontal, Value=False} {DisplayName=Flip Horizontal, Value=False} {DisplayName=Output Port - Unit 1, Value=0} {DisplayName=Output Port - Unit 2, Value=0} {DisplayName=Output Port, Value=0} {DisplayName=Exposure Time, Value=50 ms} {DisplayName=Exposure Time, Value=50 ms} {DisplayName=Scale Exposure with Binning, Value=True} {DisplayName=Scale Exposure with Binning, Value=True} {DisplayName=Binning, Value=1x1} {DisplayName=Binning, Value=1x1} {DisplayName=AOI Width, Value=2048} {DisplayName=AOI Width, Value=2048} {DisplayName=AOI Height, Value=2048} {DisplayName=AOI Height, Value=2048} {DisplayName=Data Width, Value=2048} {DisplayName=Data Width, Value=2048} {DisplayName=Data Height, Value=2048} {DisplayName=Data Height, Value=2048} {DisplayName=AOI Left, Value=1} {DisplayName=AOI Left, Value=1} {DisplayName=AOI Bottom, Value=1} {DisplayName=AOI Bottom, Value=1} {DisplayName=Overlap, Value=False} {DisplayName=Overlap, Value=False} {DisplayName=Overlap, Value=False} {DisplayName=Overlap, Value=False} {DisplayName=Low Power Mode, Value=False} {DisplayName=Bit Depth, Value=16-bit (low noise & high well capacity)} {DisplayName=Bit Depth, Value=16-bit (low noise & high well capacity)} {DisplayName=Laser Intensity 1, Value=2} {DisplayName=Laser Intensity 2, Value=40} {DisplayName=Laser Intensity 3, Value=2} {DisplayName=Laser Intensity 4, Value=2} {DisplayName=Laser Intensity 5, Value=2} {DisplayName=Laser 1, Value=False} {DisplayName=Laser 2, Value=True} {DisplayName=Laser 3, Value=False} {DisplayName=Laser 4, Value=False} {DisplayName=Laser 5, Value=False} {DisplayName=Confocal Mode, Value=0} {DisplayName=Brightfield Trigger, Value=False} {DisplayName=Brightfield Trigger Mode, Value=0} {DisplayName=Frame Event, Value=False} {DisplayName=MicroPoint Trigger Line, Value=False} {DisplayName=Spare Trigger Line, Value=False} {DisplayName=Analogue Voltage, Value=0} {DisplayName=Manual intervention needed, Value=True} {DisplayName=Ref Z, Value=77.36} {DisplayName=Drift Stabilisation Offset, Value=0} {DisplayName=Drift Stabilisation Active, Value=False} {DisplayName=Drift Stabilisation Status, Value=Off} {DisplayName=Port, Value=Left} {DisplayName=LensNumericalAperture, Value=0.15} {DisplayName=LensMagnification, Value=4} {DisplayName=ImmersionType, Value=Dry} {DisplayName=ImmersionRefractiveIndex, Value=1.0003} {DisplayName=Objective 1, Value=} {DisplayName=LensNumericalAperture, Value=0.3} {DisplayName=LensMagnification, Value=10} {DisplayName=ImmersionType, Value=Dry} {DisplayName=ImmersionRefractiveIndex, Value=1.0003} {DisplayName=Objective 2, Value=} {DisplayName=LensNumericalAperture, Value=1.05} {DisplayName=LensMagnification, Value=30} {DisplayName=ImmersionType, Value=Silicone Oil} {DisplayName=ImmersionRefractiveIndex, Value=1.406} {DisplayName=Objective 3, Value=} {DisplayName=LensNumericalAperture, Value=0.8} {DisplayName=LensMagnification, Value=40} {DisplayName=ImmersionType, Value=Water} {DisplayName=ImmersionRefractiveIndex, Value=1.3333} {DisplayName=Objective 4, Value=} {DisplayName=LensNumericalAperture, Value=1.2} {DisplayName=LensMagnification, Value=60} {DisplayName=ImmersionType, Value=Water} {DisplayName=ImmersionRefractiveIndex, Value=1.3333} {DisplayName=Objective 5, Value=} {DisplayName=LensNumericalAperture, Value=1.4} {DisplayName=LensMagnification, Value=100} {DisplayName=ImmersionType, Value=Oil} {DisplayName=ImmersionRefractiveIndex, Value=1.518} {DisplayName=Objective 6, Value=} {DisplayName=Select Objective Feature, Value=Objective 2 - contains 4 items.} {DisplayName=Objective Escaped, Value=True} {DisplayName=TotalConsolidatedOpticalMagnification, Value=10} {DisplayName=ConsolidatedImmersionRefractiveIndex, Value=1.0003} {DisplayName=ConsolidatedImmersionType, Value=Dry} {DisplayName=ConsolidatedLensNumericalAperture, Value=0.3} {DisplayName=Selected Laser Index, Value=0} {DisplayName=Laser Selector, Value=} {DisplayName=Shutter Open, Value=True} {DisplayName=Software Shuttering Mode, Value=0} {DisplayName=Frame Event, Value=False} {DisplayName=Coherence, Value=False} {DisplayName=Laser Status 1, Value=Ready} {DisplayName=Laser Status 2, Value=Ready} {DisplayName=Laser Status 3, Value=Ready} {DisplayName=Laser Status 4, Value=Ready} {DisplayName=Laser Status 5, Value=Ready} {DisplayName=Laser Type 5, Value=Laser type placeholder} {DisplayName=Laser Composite {0}1, Value=} {DisplayName=Laser Composite {0}2, Value=} {DisplayName=Laser Composite {0}3, Value=} {DisplayName=Laser Composite {0}4, Value=} {DisplayName=Laser Composite {0}5, Value=} {DisplayName=Laser Colour 1, Value=#FF5400D9} {DisplayName=Laser Wavelength 1, Value=405} {DisplayName=Laser Colour 2, Value=#FF00F5FF} {DisplayName=Laser Wavelength 2, Value=488} {DisplayName=Laser Colour 3, Value=#FFBAFF00} {DisplayName=Laser Wavelength 3, Value=561} {DisplayName=Laser Colour 4, Value=#FFFF1F00} {DisplayName=Laser Wavelength 4, Value=637} {DisplayName=Laser Colour 5, Value=#FF000000} {DisplayName=Laser Wavelength 5, Value=785} {DisplayName=Maximum Power - Unit 1, Value=250} {DisplayName=Output Power - Unit 1, Value=20} {DisplayName=Maximum Power - Unit 2, Value=250} {DisplayName=Output Power - Unit 2, Value=14} {DisplayName=Power Status, Value=0} {DisplayName=Clears interlock, Value=False} {DisplayName=Minimum Laser Excitation Wavelength, Value=488} {DisplayName=Active Blanking, Value=True} {DisplayName=Firmware Version, Value=1.37.9 - 1.37.9} {DisplayName=Magnification Correction, Value=1.0} {DisplayName=Camera Port, Value=id port 1 name [Port →]> devname [Andor Dragonfly (COM6)]} {DisplayName=Sync Exposure Time, Value=True} {DisplayName=Frame Rate, Value=0} {DisplayName=Pixel Width (µm), Value=6.5} {DisplayName=Pixel Height (µm), Value=6.5} {DisplayName=Sensor Width, Value=2048} {DisplayName=Sensor Height, Value=2048} {DisplayName=Binning X, Value=1} {DisplayName=Binning Y, Value=1} {DisplayName=Frame Averaging, Value=1} {DisplayName=HF Boost, Value=False} {DisplayName=HF Boost Filter Width, Value=1.0} {DisplayName=HF Boost Weighting, Value=0.6} {DisplayName=Readout Time (ms), Value=24.576 ms} {DisplayName=Detector Temperature, Value=-0.439999999999998} {DisplayName=Device Frame Rate, Value=13.380432455577} {DisplayName=Frame Count, Value=1} {DisplayName=Image Size Bytes, Value=8388648} {DisplayName=Sensor Cooling, Value=True} {DisplayName=Spurious Noise Filter, Value=True} {DisplayName=Metadata Enable, Value=True} {DisplayName=Metadata Timestamp, Value=True} {DisplayName=Rolling Shutter Global Clear, Value=True} {DisplayName=Auxiliary Output Source, Value=FireAll} {DisplayName=Electronic Shuttering, Value=Rolling} {DisplayName=Fan Mode, Value=On} {DisplayName=Pixel Readout Rate, Value=100 MHz} {DisplayName=Temperature Set Point, Value=0.00} {DisplayName=Temperature Status, Value=1} {DisplayName=Cycle Mode, Value=Continuous} {DisplayName=Mapping Minimum Value, Value=90} {DisplayName=Mapping Minimum Range, Value=90} {DisplayName=Advanced Acquisition Mode, Value=False} {DisplayName=Finite Burst Frame Rate, Value=2777.77778} {DisplayName=Acquisition Mode, Value=High Quality} {DisplayName=Camera Model, Value=Zyla4} {DisplayName=Is Full Sensor, Value=True} {DisplayName=Pre-defined AOI, Value=2048 x 2048} {DisplayName=Frame Latency, Value=0.074576 s} {DisplayName=Frame Wait Time, Value=5.074576 s} {DisplayName=X, Value=36.570700} {DisplayName=Y, Value=1.101800} {DisplayName=Velocity XY, Value=0, 0} {DisplayName=Maximum Absolute X Position, Value=518.9575} {DisplayName=Maximum Absolute Y Position, Value=498.9549} {DisplayName=Minimum Absolute X Position, Value=-481.0425} {DisplayName=Minimum Absolute Y Position, Value=-501.0451} {DisplayName=Maximum Bounded X Position, Value=496.7676} {DisplayName=Maximum Bounded Y Position, Value=494.4886} {DisplayName=Minimum Bounded X Position, Value=-477.4523} {DisplayName=Minimum Bounded Y Position, Value=-495.9366} {DisplayName=XY Bounded, Value=False} {DisplayName=IsTopLeftBoundSet, Value=False} {DisplayName=IsBottomRightBoundSet, Value=False} {DisplayName=Wait Until Movement Stopped, Value=False} {DisplayName=Invert Software X Axis, Value=True} {DisplayName=X Resolution, Value=0.01} {DisplayName=Speed Limit X, Value=5.745920} {DisplayName=Invert Software Y Axis, Value=True} {DisplayName=Y Resolution, Value=0.01} {DisplayName=Speed Limit Y, Value=5.745920} {DisplayName=Settling Time, Value=15 ms} {DisplayName=Z, Value=134.0} {DisplayName=Invert Software Z Axis, Value=False} {DisplayName=Z Resolution, Value=0.01} {DisplayName=Speed Limit Z, Value=600.320000} {DisplayName=Velocity Z, Value=0.000000} {DisplayName=Baud Rate, Value=9600} {DisplayName=Magnification Correction, Value=1.0} {DisplayName=Camera Port, Value=id port 2 name [Port ↑]> devname [Andor Dragonfly (COM6)]} {DisplayName=Sync Exposure Time, Value=True} {DisplayName=Frame Rate, Value=0} {DisplayName=Pixel Width (µm), Value=6.5} {DisplayName=Pixel Height (µm), Value=6.5} {DisplayName=Sensor Width, Value=2048} {DisplayName=Sensor Height, Value=2048} {DisplayName=Binning X, Value=1} {DisplayName=Binning Y, Value=1} {DisplayName=Frame Averaging, Value=1} {DisplayName=HF Boost, Value=False} {DisplayName=HF Boost Filter Width, Value=1.0} {DisplayName=HF Boost Weighting, Value=0.6} {DisplayName=Readout Time (ms), Value=24.576 ms} {DisplayName=Detector Temperature, Value=-0.439999999999998} {DisplayName=Device Frame Rate, Value=13.380432455577} {DisplayName=Frame Count, Value=1} {DisplayName=Image Size Bytes, Value=8388648} {DisplayName=Sensor Cooling, Value=True} {DisplayName=Spurious Noise Filter, Value=True} {DisplayName=Metadata Enable, Value=True} {DisplayName=Metadata Timestamp, Value=True} {DisplayName=Rolling Shutter Global Clear, Value=True} {DisplayName=Auxiliary Output Source, Value=FireAll} {DisplayName=Electronic Shuttering, Value=Rolling} {DisplayName=Fan Mode, Value=On} {DisplayName=Pixel Readout Rate, Value=100 MHz} {DisplayName=Temperature Set Point, Value=0.00} {DisplayName=Temperature Status, Value=1} {DisplayName=Cycle Mode, Value=Continuous} {DisplayName=Mapping Minimum Value, Value=90} {DisplayName=Mapping Minimum Range, Value=90} {DisplayName=Advanced Acquisition Mode, Value=False} {DisplayName=Finite Burst Frame Rate, Value=2777.77778} {DisplayName=Acquisition Mode, Value=High Quality} {DisplayName=Camera Model, Value=Zyla4} {DisplayName=Is Full Sensor, Value=True} {DisplayName=Pre-defined AOI, Value=2048 x 2048} {DisplayName=Frame Latency, Value=0.074576 s} {DisplayName=Frame Wait Time, Value=5.074576 s} {DisplayName=Disk Rotation, Value=True} {DisplayName=Actual Disk Speed, Value=5988} {DisplayName=Disk Speed, Value=6000} {DisplayName=Dichroic Mirror, Value=0} {DisplayName=Image Splitter, Value=1} {DisplayName=Filter Wheel →, Value=1} {DisplayName=Filter Wheel ↑, Value=1} {DisplayName=Camera Magnification →, Value=0} {DisplayName=Camera Magnification ↑, Value=0} {DisplayName=Camera Port, Value=2} {DisplayName=TotalConsolidatedOpticalMagnification, Value=1.078} {DisplayName=Magnification Correction, Value=1.078} {DisplayName=Illumination Aperture, Value=1} {DisplayName=Pinhole Size, Value=40} {DisplayName=Number Of Scan Per Revolution, Value=4} {DisplayName=Filter Wheel Mode, Value=0} {DisplayName=Serial Number, Value=DFLY-0277} {DisplayName=Product ID, Value=202-2540} {DisplayName=Firmware Version, Value=1.35} {DisplayName=LensNumericalAperture, Value=1.4} {DisplayName=LensMagnification, Value=100} {DisplayName=ImmersionType, Value=Oil} {DisplayName=ImmersionRefractiveIndex, Value=1.518} {DisplayName=Objective 7, Value=} {DisplayName=Fast AOI Frame Rate Enable, Value=True} {DisplayName=End Of Exposure Enabled, Value=True} {DisplayName=Fast AOI Frame Rate Enable, Value=True} {DisplayName=End Of Exposure Enabled, Value=True} {DisplayName=Analogue Control, Value=False} {DisplayName=Device Trigger Mode, Value=Software} {DisplayName=Trigger Mode, Value=0} {DisplayName=External Trigger Timeout, Value=10 s} {DisplayName=Simulated Global Shutter, Value=False} {DisplayName=Simulated Global Shutter, Value=False} {DisplayName=Device Trigger Mode, Value=Software} {DisplayName=Trigger Mode, Value=0} {DisplayName=External Trigger Timeout, Value=10 s} IsOnDisk=True IsValid=True [Peripherals] [Channel] Wavelength=698 nm IsPrimaryChannel=True AutoGenerateColour=False EIRMode=Full AcquisitionDeviceAlias=Zyla 1 ImagingMode={Name=CF40} Name=637_Cy5_CF40_Zyla 1 Description= IsActive=False IsReadOnly=False [FeatureValueList] {DisplayName=Rotation, Value=0˚} {DisplayName=Rotation, Value=0˚} {DisplayName=Flip Vertical, Value=True} {DisplayName=Flip Vertical, Value=True} {DisplayName=Flip Horizontal, Value=False} {DisplayName=Flip Horizontal, Value=False} {DisplayName=Output Port - Unit 1, Value=0} {DisplayName=Output Port - Unit 2, Value=0} {DisplayName=Output Port, Value=0} {DisplayName=Exposure Time, Value=50 ms} {DisplayName=Exposure Time, Value=200 ms} {DisplayName=Scale Exposure with Binning, Value=True} {DisplayName=Scale Exposure with Binning, Value=True} {DisplayName=Binning, Value=1x1} {DisplayName=Binning, Value=1x1} {DisplayName=AOI Width, Value=2048} {DisplayName=AOI Width, Value=2048} {DisplayName=AOI Height, Value=2048} {DisplayName=AOI Height, Value=2048} {DisplayName=Data Width, Value=2048} {DisplayName=Data Width, Value=2048} {DisplayName=Data Height, Value=2048} {DisplayName=Data Height, Value=2048} {DisplayName=AOI Left, Value=1} {DisplayName=AOI Left, Value=1} {DisplayName=AOI Bottom, Value=1} {DisplayName=AOI Bottom, Value=1} {DisplayName=Overlap, Value=False} {DisplayName=Overlap, Value=False} {DisplayName=Overlap, Value=False} {DisplayName=Overlap, Value=False} {DisplayName=Low Power Mode, Value=False} {DisplayName=Bit Depth, Value=16-bit (low noise & high well capacity)} {DisplayName=Bit Depth, Value=16-bit (low noise & high well capacity)} {DisplayName=Laser Intensity 1, Value=2} {DisplayName=Laser Intensity 2, Value=2} {DisplayName=Laser Intensity 3, Value=19} {DisplayName=Laser Intensity 4, Value=15} {DisplayName=Laser Intensity 5, Value=2} {DisplayName=Laser 1, Value=False} {DisplayName=Laser 2, Value=False} {DisplayName=Laser 3, Value=False} {DisplayName=Laser 4, Value=True} {DisplayName=Laser 5, Value=False} {DisplayName=Confocal Mode, Value=0} {DisplayName=Brightfield Trigger, Value=False} {DisplayName=Brightfield Trigger Mode, Value=0} {DisplayName=Frame Event, Value=False} {DisplayName=MicroPoint Trigger Line, Value=False} {DisplayName=Spare Trigger Line, Value=False} {DisplayName=Analogue Voltage, Value=0} {DisplayName=Manual intervention needed, Value=True} {DisplayName=Ref Z, Value=77.36} {DisplayName=Drift Stabilisation Offset, Value=0} {DisplayName=Drift Stabilisation Active, Value=False} {DisplayName=Drift Stabilisation Status, Value=Off} {DisplayName=Port, Value=Left} {DisplayName=LensNumericalAperture, Value=0.15} {DisplayName=LensMagnification, Value=4} {DisplayName=ImmersionType, Value=Dry} {DisplayName=ImmersionRefractiveIndex, Value=1.0003} {DisplayName=Objective 1, Value=} {DisplayName=LensNumericalAperture, Value=0.3} {DisplayName=LensMagnification, Value=10} {DisplayName=ImmersionType, Value=Dry} {DisplayName=ImmersionRefractiveIndex, Value=1.0003} {DisplayName=Objective 2, Value=} {DisplayName=LensNumericalAperture, Value=1.05} {DisplayName=LensMagnification, Value=30} {DisplayName=ImmersionType, Value=Silicone Oil} {DisplayName=ImmersionRefractiveIndex, Value=1.406} {DisplayName=Objective 3, Value=} {DisplayName=LensNumericalAperture, Value=0.8} {DisplayName=LensMagnification, Value=40} {DisplayName=ImmersionType, Value=Water} {DisplayName=ImmersionRefractiveIndex, Value=1.3333} {DisplayName=Objective 4, Value=} {DisplayName=LensNumericalAperture, Value=1.2} {DisplayName=LensMagnification, Value=60} {DisplayName=ImmersionType, Value=Water} {DisplayName=ImmersionRefractiveIndex, Value=1.3333} {DisplayName=Objective 5, Value=} {DisplayName=LensNumericalAperture, Value=1.4} {DisplayName=LensMagnification, Value=100} {DisplayName=ImmersionType, Value=Oil} {DisplayName=ImmersionRefractiveIndex, Value=1.518} {DisplayName=Objective 6, Value=} {DisplayName=Select Objective Feature, Value=Objective 2 - contains 4 items.} {DisplayName=Objective Escaped, Value=True} {DisplayName=TotalConsolidatedOpticalMagnification, Value=10} {DisplayName=ConsolidatedImmersionRefractiveIndex, Value=1.0003} {DisplayName=ConsolidatedImmersionType, Value=Dry} {DisplayName=ConsolidatedLensNumericalAperture, Value=0.3} {DisplayName=Selected Laser Index, Value=0} {DisplayName=Laser Selector, Value=} {DisplayName=Shutter Open, Value=True} {DisplayName=Software Shuttering Mode, Value=0} {DisplayName=Frame Event, Value=False} {DisplayName=Coherence, Value=False} {DisplayName=Laser Status 1, Value=Ready} {DisplayName=Laser Status 2, Value=Ready} {DisplayName=Laser Status 3, Value=Ready} {DisplayName=Laser Status 4, Value=Ready} {DisplayName=Laser Status 5, Value=Ready} {DisplayName=Laser Type 5, Value=Laser type placeholder} {DisplayName=Laser Composite {0}1, Value=} {DisplayName=Laser Composite {0}2, Value=} {DisplayName=Laser Composite {0}3, Value=} {DisplayName=Laser Composite {0}4, Value=} {DisplayName=Laser Composite {0}5, Value=} {DisplayName=Laser Colour 1, Value=#FF5400D9} {DisplayName=Laser Wavelength 1, Value=405} {DisplayName=Laser Colour 2, Value=#FF00F5FF} {DisplayName=Laser Wavelength 2, Value=488} {DisplayName=Laser Colour 3, Value=#FFBAFF00} {DisplayName=Laser Wavelength 3, Value=561} {DisplayName=Laser Colour 4, Value=#FFFF1F00} {DisplayName=Laser Wavelength 4, Value=637} {DisplayName=Laser Colour 5, Value=#FF000000} {DisplayName=Laser Wavelength 5, Value=785} {DisplayName=Maximum Power - Unit 1, Value=250} {DisplayName=Output Power - Unit 1, Value=20} {DisplayName=Maximum Power - Unit 2, Value=250} {DisplayName=Output Power - Unit 2, Value=14} {DisplayName=Power Status, Value=0} {DisplayName=Clears interlock, Value=False} {DisplayName=Minimum Laser Excitation Wavelength, Value=637} {DisplayName=Active Blanking, Value=True} {DisplayName=Firmware Version, Value=1.37.9 - 1.37.9} {DisplayName=Magnification Correction, Value=1.0} {DisplayName=Camera Port, Value=id port 1 name [Port →]> devname [Andor Dragonfly (COM6)]} {DisplayName=Sync Exposure Time, Value=True} {DisplayName=Frame Rate, Value=0} {DisplayName=Pixel Width (µm), Value=6.5} {DisplayName=Pixel Height (µm), Value=6.5} {DisplayName=Sensor Width, Value=2048} {DisplayName=Sensor Height, Value=2048} {DisplayName=Binning X, Value=1} {DisplayName=Binning Y, Value=1} {DisplayName=Frame Averaging, Value=1} {DisplayName=HF Boost, Value=False} {DisplayName=HF Boost Filter Width, Value=1.0} {DisplayName=HF Boost Weighting, Value=0.6} {DisplayName=Readout Time (ms), Value=24.576 ms} {DisplayName=Detector Temperature, Value=-0.439999999999998} {DisplayName=Device Frame Rate, Value=13.380432455577} {DisplayName=Frame Count, Value=1} {DisplayName=Image Size Bytes, Value=8388648} {DisplayName=Sensor Cooling, Value=True} {DisplayName=Spurious Noise Filter, Value=True} {DisplayName=Metadata Enable, Value=True} {DisplayName=Metadata Timestamp, Value=True} {DisplayName=Rolling Shutter Global Clear, Value=True} {DisplayName=Auxiliary Output Source, Value=FireAll} {DisplayName=Electronic Shuttering, Value=Rolling} {DisplayName=Fan Mode, Value=On} {DisplayName=Pixel Readout Rate, Value=100 MHz} {DisplayName=Temperature Set Point, Value=0.00} {DisplayName=Temperature Status, Value=1} {DisplayName=Cycle Mode, Value=Continuous} {DisplayName=Mapping Minimum Value, Value=90} {DisplayName=Mapping Minimum Range, Value=90} {DisplayName=Advanced Acquisition Mode, Value=False} {DisplayName=Finite Burst Frame Rate, Value=2777.77778} {DisplayName=Acquisition Mode, Value=High Quality} {DisplayName=Camera Model, Value=Zyla4} {DisplayName=Is Full Sensor, Value=True} {DisplayName=Pre-defined AOI, Value=2048 x 2048} {DisplayName=Frame Latency, Value=0.074576 s} {DisplayName=Frame Wait Time, Value=5.074576 s} {DisplayName=X, Value=36.570700} {DisplayName=Y, Value=1.101800} {DisplayName=Velocity XY, Value=0, 0} {DisplayName=Maximum Absolute X Position, Value=518.9575} {DisplayName=Maximum Absolute Y Position, Value=498.9549} {DisplayName=Minimum Absolute X Position, Value=-481.0425} {DisplayName=Minimum Absolute Y Position, Value=-501.0451} {DisplayName=Maximum Bounded X Position, Value=496.7676} {DisplayName=Maximum Bounded Y Position, Value=494.4886} {DisplayName=Minimum Bounded X Position, Value=-477.4523} {DisplayName=Minimum Bounded Y Position, Value=-495.9366} {DisplayName=XY Bounded, Value=False} {DisplayName=IsTopLeftBoundSet, Value=False} {DisplayName=IsBottomRightBoundSet, Value=False} {DisplayName=Wait Until Movement Stopped, Value=False} {DisplayName=Invert Software X Axis, Value=True} {DisplayName=X Resolution, Value=0.01} {DisplayName=Speed Limit X, Value=5.745920} {DisplayName=Invert Software Y Axis, Value=True} {DisplayName=Y Resolution, Value=0.01} {DisplayName=Speed Limit Y, Value=5.745920} {DisplayName=Settling Time, Value=15 ms} {DisplayName=Z, Value=134.0} {DisplayName=Invert Software Z Axis, Value=False} {DisplayName=Z Resolution, Value=0.01} {DisplayName=Speed Limit Z, Value=600.320000} {DisplayName=Velocity Z, Value=0.000000} {DisplayName=Baud Rate, Value=9600} {DisplayName=Magnification Correction, Value=1.0} {DisplayName=Camera Port, Value=id port 2 name [Port ↑]> devname [Andor Dragonfly (COM6)]} {DisplayName=Sync Exposure Time, Value=True} {DisplayName=Frame Rate, Value=0} {DisplayName=Pixel Width (µm), Value=6.5} {DisplayName=Pixel Height (µm), Value=6.5} {DisplayName=Sensor Width, Value=2048} {DisplayName=Sensor Height, Value=2048} {DisplayName=Binning X, Value=1} {DisplayName=Binning Y, Value=1} {DisplayName=Frame Averaging, Value=1} {DisplayName=HF Boost, Value=False} {DisplayName=HF Boost Filter Width, Value=1.0} {DisplayName=HF Boost Weighting, Value=0.6} {DisplayName=Readout Time (ms), Value=24.576 ms} {DisplayName=Detector Temperature, Value=-0.439999999999998} {DisplayName=Device Frame Rate, Value=13.380432455577} {DisplayName=Frame Count, Value=1} {DisplayName=Image Size Bytes, Value=8388648} {DisplayName=Sensor Cooling, Value=True} {DisplayName=Spurious Noise Filter, Value=True} {DisplayName=Metadata Enable, Value=True} {DisplayName=Metadata Timestamp, Value=True} {DisplayName=Rolling Shutter Global Clear, Value=True} {DisplayName=Auxiliary Output Source, Value=FireAll} {DisplayName=Electronic Shuttering, Value=Rolling} {DisplayName=Fan Mode, Value=On} {DisplayName=Pixel Readout Rate, Value=100 MHz} {DisplayName=Temperature Set Point, Value=0.00} {DisplayName=Temperature Status, Value=1} {DisplayName=Cycle Mode, Value=Continuous} {DisplayName=Mapping Minimum Value, Value=90} {DisplayName=Mapping Minimum Range, Value=90} {DisplayName=Advanced Acquisition Mode, Value=False} {DisplayName=Finite Burst Frame Rate, Value=2777.77778} {DisplayName=Acquisition Mode, Value=High Quality} {DisplayName=Camera Model, Value=Zyla4} {DisplayName=Is Full Sensor, Value=True} {DisplayName=Pre-defined AOI, Value=2048 x 2048} {DisplayName=Frame Latency, Value=0.224576 s} {DisplayName=Frame Wait Time, Value=5.074576 s} {DisplayName=Disk Rotation, Value=True} {DisplayName=Actual Disk Speed, Value=5988} {DisplayName=Disk Speed, Value=6000} {DisplayName=Dichroic Mirror, Value=0} {DisplayName=Image Splitter, Value=1} {DisplayName=Filter Wheel →, Value=1} {DisplayName=Filter Wheel ↑, Value=1} {DisplayName=Camera Magnification →, Value=0} {DisplayName=Camera Magnification ↑, Value=0} {DisplayName=Camera Port, Value=2} {DisplayName=TotalConsolidatedOpticalMagnification, Value=1.078} {DisplayName=Magnification Correction, Value=1.078} {DisplayName=Illumination Aperture, Value=1} {DisplayName=Pinhole Size, Value=40} {DisplayName=Number Of Scan Per Revolution, Value=4} {DisplayName=Filter Wheel Mode, Value=0} {DisplayName=Serial Number, Value=DFLY-0277} {DisplayName=Product ID, Value=202-2540} {DisplayName=Firmware Version, Value=1.35} {DisplayName=LensNumericalAperture, Value=1.4} {DisplayName=LensMagnification, Value=100} {DisplayName=ImmersionType, Value=Oil} {DisplayName=ImmersionRefractiveIndex, Value=1.518} {DisplayName=Objective 7, Value=} {DisplayName=Fast AOI Frame Rate Enable, Value=True} {DisplayName=End Of Exposure Enabled, Value=True} {DisplayName=Fast AOI Frame Rate Enable, Value=True} {DisplayName=End Of Exposure Enabled, Value=True} {DisplayName=Analogue Control, Value=False} {DisplayName=Device Trigger Mode, Value=Software} {DisplayName=Trigger Mode, Value=0} {DisplayName=External Trigger Timeout, Value=10 s} {DisplayName=Simulated Global Shutter, Value=False} {DisplayName=Simulated Global Shutter, Value=False} {DisplayName=Device Trigger Mode, Value=Software} {DisplayName=Trigger Mode, Value=0} {DisplayName=External Trigger Timeout, Value=10 s} IsOnDisk=True IsValid=True IsValid=True [TriggeringProtocolSpecification] IsViewExpanded=False IsValid=True [Imaging Modes In Protocol] [ImagingMode] Modality=SpinningDiskConfocal IsConfigurable=True IsChannelAssignable=True Name=CF40 Description= IsActive=True IsReadOnly=True [FeatureValueList] {DisplayName=Rotation, Value=0˚} {DisplayName=Rotation, Value=0˚} {DisplayName=Flip Vertical, Value=True} {DisplayName=Flip Vertical, Value=True} {DisplayName=Flip Horizontal, Value=False} {DisplayName=Flip Horizontal, Value=False} {DisplayName=Output Port - Unit 1, Value=0} {DisplayName=Output Port - Unit 2, Value=0} {DisplayName=Output Port, Value=0} {DisplayName=Exposure Time, Value=50 ms} {DisplayName=Exposure Time, Value=50 ms} {DisplayName=Scale Exposure with Binning, Value=True} {DisplayName=Scale Exposure with Binning, Value=True} {DisplayName=Binning, Value=1x1} {DisplayName=Binning, Value=1x1} {DisplayName=AOI Width, Value=2048} {DisplayName=AOI Width, Value=2048} {DisplayName=AOI Height, Value=2048} {DisplayName=AOI Height, Value=2048} {DisplayName=Data Width, Value=2048} {DisplayName=Data Width, Value=2048} {DisplayName=Data Height, Value=2048} {DisplayName=Data Height, Value=2048} {DisplayName=AOI Left, Value=1} {DisplayName=AOI Left, Value=1} {DisplayName=AOI Bottom, Value=1} {DisplayName=AOI Bottom, Value=1} {DisplayName=Overlap, Value=False} {DisplayName=Overlap, Value=False} {DisplayName=Overlap, Value=False} {DisplayName=Overlap, Value=False} {DisplayName=Bit Depth, Value=16-bit (low noise & high well capacity)} {DisplayName=Low Power Mode, Value=False} {DisplayName=Bit Depth, Value=16-bit (low noise & high well capacity)} {DisplayName=Laser Intensity 1, Value=2} {DisplayName=Laser Intensity 2, Value=40} {DisplayName=Laser Intensity 3, Value=2} {DisplayName=Laser Intensity 4, Value=2} {DisplayName=Laser Intensity 5, Value=2} {DisplayName=Laser 1, Value=False} {DisplayName=Laser 2, Value=True} {DisplayName=Laser 3, Value=False} {DisplayName=Laser 4, Value=False} {DisplayName=Laser 5, Value=False} {DisplayName=Confocal Mode, Value=0} {DisplayName=Magnification Correction, Value=1.0} {DisplayName=Camera Port, Value=id port 1 name [Port →]> devname [Andor Dragonfly (COM6)]} {DisplayName=Sync Exposure Time, Value=True} {DisplayName=Frame Rate, Value=0} {DisplayName=Pixel Width (µm), Value=6.5} {DisplayName=Pixel Height (µm), Value=6.5} {DisplayName=Sensor Width, Value=2048} {DisplayName=Sensor Height, Value=2048} {DisplayName=Binning X, Value=1} {DisplayName=Binning Y, Value=1} {DisplayName=Frame Averaging, Value=1} {DisplayName=HF Boost, Value=False} {DisplayName=HF Boost Filter Width, Value=1.0} {DisplayName=HF Boost Weighting, Value=0.6} {DisplayName=Readout Time (ms), Value=24.576 ms} {DisplayName=Detector Temperature, Value=-0.439999999999998} {DisplayName=Device Frame Rate, Value=13.380432455577} {DisplayName=Frame Count, Value=1} {DisplayName=Image Size Bytes, Value=8388648} {DisplayName=Sensor Cooling, Value=True} {DisplayName=Spurious Noise Filter, Value=True} {DisplayName=Metadata Enable, Value=True} {DisplayName=Metadata Timestamp, Value=True} {DisplayName=Rolling Shutter Global Clear, Value=True} {DisplayName=Auxiliary Output Source, Value=FireAll} {DisplayName=Electronic Shuttering, Value=Rolling} {DisplayName=Fan Mode, Value=On} {DisplayName=Pixel Readout Rate, Value=100 MHz} {DisplayName=Temperature Set Point, Value=0.00} {DisplayName=Temperature Status, Value=1} {DisplayName=Cycle Mode, Value=Continuous} {DisplayName=Mapping Minimum Value, Value=90} {DisplayName=Mapping Minimum Range, Value=90} {DisplayName=Advanced Acquisition Mode, Value=False} {DisplayName=Finite Burst Frame Rate, Value=2777.77778} {DisplayName=Acquisition Mode, Value=High Quality} {DisplayName=Camera Model, Value=Zyla4} {DisplayName=Is Full Sensor, Value=True} {DisplayName=Pre-defined AOI, Value=2048 x 2048} {DisplayName=Frame Latency, Value=0.074576 s} {DisplayName=Frame Wait Time, Value=5.074576 s} {DisplayName=Selected Laser Index, Value=0} {DisplayName=Laser Selector, Value=} {DisplayName=Shutter Open, Value=True} {DisplayName=Software Shuttering Mode, Value=0} {DisplayName=Frame Event, Value=False} {DisplayName=Coherence, Value=False} {DisplayName=Laser Status 1, Value=Ready} {DisplayName=Laser Status 2, Value=Ready} {DisplayName=Laser Status 3, Value=Ready} {DisplayName=Laser Status 4, Value=Ready} {DisplayName=Laser Status 5, Value=Ready} {DisplayName=Laser Type 5, Value=Laser type placeholder} {DisplayName=Laser Composite {0}1, Value=} {DisplayName=Laser Composite {0}2, Value=} {DisplayName=Laser Composite {0}3, Value=} {DisplayName=Laser Composite {0}4, Value=} {DisplayName=Laser Composite {0}5, Value=} {DisplayName=Laser Colour 1, Value=#FF5400D9} {DisplayName=Laser Wavelength 1, Value=405} {DisplayName=Laser Colour 2, Value=#FF00F5FF} {DisplayName=Laser Wavelength 2, Value=488} {DisplayName=Laser Colour 3, Value=#FFBAFF00} {DisplayName=Laser Wavelength 3, Value=561} {DisplayName=Laser Colour 4, Value=#FFFF1F00} {DisplayName=Laser Wavelength 4, Value=637} {DisplayName=Laser Colour 5, Value=#FF000000} {DisplayName=Laser Wavelength 5, Value=785} {DisplayName=Maximum Power - Unit 1, Value=250} {DisplayName=Output Power - Unit 1, Value=20} {DisplayName=Maximum Power - Unit 2, Value=250} {DisplayName=Output Power - Unit 2, Value=14} {DisplayName=Power Status, Value=0} {DisplayName=Clears interlock, Value=False} {DisplayName=Minimum Laser Excitation Wavelength, Value=488} {DisplayName=Active Blanking, Value=True} {DisplayName=Firmware Version, Value=1.37.9 - 1.37.9} {DisplayName=Disk Rotation, Value=True} {DisplayName=Actual Disk Speed, Value=5999} {DisplayName=Disk Speed, Value=6000} {DisplayName=Dichroic Mirror, Value=0} {DisplayName=Image Splitter, Value=1} {DisplayName=Filter Wheel →, Value=1} {DisplayName=Filter Wheel ↑, Value=1} {DisplayName=Camera Magnification →, Value=0} {DisplayName=Camera Magnification ↑, Value=0} {DisplayName=Camera Port, Value=2} {DisplayName=TotalConsolidatedOpticalMagnification, Value=1.078} {DisplayName=Magnification Correction, Value=1.078} {DisplayName=Illumination Aperture, Value=1} {DisplayName=Pinhole Size, Value=40} {DisplayName=Number Of Scan Per Revolution, Value=4} {DisplayName=Filter Wheel Mode, Value=0} {DisplayName=Serial Number, Value=DFLY-0277} {DisplayName=Product ID, Value=202-2540} {DisplayName=Firmware Version, Value=1.35} {DisplayName=X, Value=36.570700} {DisplayName=Y, Value=1.101800} {DisplayName=Velocity XY, Value=0, 0} {DisplayName=Maximum Absolute X Position, Value=518.9575} {DisplayName=Maximum Absolute Y Position, Value=498.9549} {DisplayName=Minimum Absolute X Position, Value=-481.0425} {DisplayName=Minimum Absolute Y Position, Value=-501.0451} {DisplayName=Maximum Bounded X Position, Value=496.7676} {DisplayName=Maximum Bounded Y Position, Value=494.4886} {DisplayName=Minimum Bounded X Position, Value=-477.4523} {DisplayName=Minimum Bounded Y Position, Value=-495.9366} {DisplayName=XY Bounded, Value=False} {DisplayName=IsTopLeftBoundSet, Value=False} {DisplayName=IsBottomRightBoundSet, Value=False} {DisplayName=Wait Until Movement Stopped, Value=False} {DisplayName=Invert Software X Axis, Value=True} {DisplayName=X Resolution, Value=0.01} {DisplayName=Speed Limit X, Value=5.745920} {DisplayName=Invert Software Y Axis, Value=True} {DisplayName=Y Resolution, Value=0.01} {DisplayName=Speed Limit Y, Value=5.745920} {DisplayName=Settling Time, Value=15 ms} {DisplayName=Z, Value=134.0} {DisplayName=Invert Software Z Axis, Value=False} {DisplayName=Z Resolution, Value=0.01} {DisplayName=Speed Limit Z, Value=600.320000} {DisplayName=Velocity Z, Value=0.000000} {DisplayName=Baud Rate, Value=9600} {DisplayName=Magnification Correction, Value=1.0} {DisplayName=Camera Port, Value=id port 2 name [Port ↑]> devname [Andor Dragonfly (COM6)]} {DisplayName=Sync Exposure Time, Value=True} {DisplayName=Frame Rate, Value=0} {DisplayName=Pixel Width (µm), Value=6.5} {DisplayName=Pixel Height (µm), Value=6.5} {DisplayName=Sensor Width, Value=2048} {DisplayName=Sensor Height, Value=2048} {DisplayName=Binning X, Value=1} {DisplayName=Binning Y, Value=1} {DisplayName=Frame Averaging, Value=1} {DisplayName=HF Boost, Value=False} {DisplayName=HF Boost Filter Width, Value=1.0} {DisplayName=HF Boost Weighting, Value=0.6} {DisplayName=Readout Time (ms), Value=24.576 ms} {DisplayName=Detector Temperature, Value=-0.439999999999998} {DisplayName=Device Frame Rate, Value=13.380432455577} {DisplayName=Frame Count, Value=1} {DisplayName=Image Size Bytes, Value=8388648} {DisplayName=Sensor Cooling, Value=True} {DisplayName=Spurious Noise Filter, Value=True} {DisplayName=Metadata Enable, Value=True} {DisplayName=Metadata Timestamp, Value=True} {DisplayName=Rolling Shutter Global Clear, Value=True} {DisplayName=Auxiliary Output Source, Value=FireAll} {DisplayName=Electronic Shuttering, Value=Rolling} {DisplayName=Fan Mode, Value=On} {DisplayName=Pixel Readout Rate, Value=100 MHz} {DisplayName=Temperature Set Point, Value=0.00} {DisplayName=Temperature Status, Value=1} {DisplayName=Cycle Mode, Value=Continuous} {DisplayName=Mapping Minimum Value, Value=90} {DisplayName=Mapping Minimum Range, Value=90} {DisplayName=Advanced Acquisition Mode, Value=False} {DisplayName=Finite Burst Frame Rate, Value=2777.77778} {DisplayName=Acquisition Mode, Value=High Quality} {DisplayName=Camera Model, Value=Zyla4} {DisplayName=Is Full Sensor, Value=True} {DisplayName=Pre-defined AOI, Value=2048 x 2048} {DisplayName=Frame Latency, Value=0.074576 s} {DisplayName=Frame Wait Time, Value=5.074576 s} {DisplayName=Brightfield Trigger, Value=False} {DisplayName=Brightfield Trigger Mode, Value=0} {DisplayName=Frame Event, Value=False} {DisplayName=MicroPoint Trigger Line, Value=False} {DisplayName=Spare Trigger Line, Value=False} {DisplayName=Analogue Voltage, Value=0} {DisplayName=Manual intervention needed, Value=True} {DisplayName=Ref Z, Value=77.36} {DisplayName=Drift Stabilisation Offset, Value=0} {DisplayName=Drift Stabilisation Active, Value=False} {DisplayName=Drift Stabilisation Status, Value=Off} {DisplayName=Port, Value=Left} {DisplayName=LensNumericalAperture, Value=0.15} {DisplayName=LensMagnification, Value=4} {DisplayName=ImmersionType, Value=Dry} {DisplayName=ImmersionRefractiveIndex, Value=1.0003} {DisplayName=Objective 1, Value=} {DisplayName=LensNumericalAperture, Value=0.3} {DisplayName=LensMagnification, Value=10} {DisplayName=ImmersionType, Value=Dry} {DisplayName=ImmersionRefractiveIndex, Value=1.0003} {DisplayName=Objective 2, Value=} {DisplayName=LensNumericalAperture, Value=1.05} {DisplayName=LensMagnification, Value=30} {DisplayName=ImmersionType, Value=Silicone Oil} {DisplayName=ImmersionRefractiveIndex, Value=1.406} {DisplayName=Objective 3, Value=} {DisplayName=LensNumericalAperture, Value=0.8} {DisplayName=LensMagnification, Value=40} {DisplayName=ImmersionType, Value=Water} {DisplayName=ImmersionRefractiveIndex, Value=1.3333} {DisplayName=Objective 4, Value=} {DisplayName=LensNumericalAperture, Value=1.2} {DisplayName=LensMagnification, Value=60} {DisplayName=ImmersionType, Value=Water} {DisplayName=ImmersionRefractiveIndex, Value=1.3333} {DisplayName=Objective 5, Value=} {DisplayName=LensNumericalAperture, Value=1.4} {DisplayName=LensMagnification, Value=100} {DisplayName=ImmersionType, Value=Oil} {DisplayName=ImmersionRefractiveIndex, Value=1.518} {DisplayName=Objective 6, Value=} {DisplayName=Select Objective Feature, Value=Objective 2 - contains 4 items.} {DisplayName=Objective Escaped, Value=True} {DisplayName=TotalConsolidatedOpticalMagnification, Value=10} {DisplayName=ConsolidatedImmersionRefractiveIndex, Value=1.0003} {DisplayName=ConsolidatedImmersionType, Value=Dry} {DisplayName=ConsolidatedLensNumericalAperture, Value=0.3} {DisplayName=LensNumericalAperture, Value=1.4} {DisplayName=LensMagnification, Value=100} {DisplayName=ImmersionType, Value=Oil} {DisplayName=ImmersionRefractiveIndex, Value=1.518} {DisplayName=Objective 7, Value=} {DisplayName=Fast AOI Frame Rate Enable, Value=True} {DisplayName=End Of Exposure Enabled, Value=True} {DisplayName=Fast AOI Frame Rate Enable, Value=True} {DisplayName=End Of Exposure Enabled, Value=True} {DisplayName=Analogue Control, Value=False} {DisplayName=Device Trigger Mode, Value=Software} {DisplayName=Trigger Mode, Value=0} {DisplayName=External Trigger Timeout, Value=10 s} {DisplayName=Device Trigger Mode, Value=Software} {DisplayName=Trigger Mode, Value=0} {DisplayName=External Trigger Timeout, Value=10 s} {DisplayName=Simulated Global Shutter, Value=False} {DisplayName=Simulated Global Shutter, Value=False} IsOnDisk=True IsValid=True [Protocol Options Sections] [ProtocolDeconvolutionOptions] Name=DeconvolutionOptions Apply3DDeconvolution=False ProcessOnlyDuringProtocolTimeIntervals=False ProcessDuringProtocolTimeIntervalsOrFrameAcquisitions=True IsValid=True [ProtocolStitchingOptions] StitchingMethod=PhaseCorrelation Invert=False BackgroundSubtraction=True FilterWidth=0 UseSpecifiedMode=False Name=StitchingOptions ApplyStitching=False StitchingMode=HighSpeed ChannelIndex=0 IsValid=True [ProtocolLoopOptions] Name=LoopOptions IsLoopContinuously=False NumberOfLoops=1 ProtocolInterval=0 s IsValid=True [Events]