{
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"FrameKey-0-0-0": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "520",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 153.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "5.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 640620,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 0,
"SlicePosition": -50,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "565/24(G)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 0,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 320,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-G_000.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "320.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:23 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 0,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "2fb219c1-3260-40a0-a78e-a534f16e448c",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-G",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "1",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "4.5000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-0-1": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "520",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 163.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "5.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 641241,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 1,
"SlicePosition": -40,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "565/24(G)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 0,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 320,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-G_001.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "320.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:23 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 1,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "fd2f136f-58cf-44da-81a3-3bcd6962c178",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-G",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "1",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "4.5000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-0-2": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "520",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 173.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "5.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 641795,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 2,
"SlicePosition": -30,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "565/24(G)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 0,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 320,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-G_002.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "320.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:24 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 2,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "cbe970cc-28d4-42b1-b3c2-5a9a2accc47a",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-G",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "1",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "4.5000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-0-3": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "520",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 183.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "5.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 642359,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 3,
"SlicePosition": -20,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "565/24(G)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 0,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 320,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-G_003.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "320.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:25 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 3,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "52866869-7e7c-4503-9775-6f38b0dbb035",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-G",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "1",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "4.5000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-0-4": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "520",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 193.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "5.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 642918,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 4,
"SlicePosition": -10,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "565/24(G)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 0,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 320,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-G_004.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "320.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:25 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 4,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "2ac52d1e-5bf1-41a9-ad31-9aa1226bed26",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-G",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "1",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "4.5000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-0-5": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "520",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 203.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "5.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 643476,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 5,
"SlicePosition": 0,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "565/24(G)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 0,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 320,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-G_005.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "320.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:26 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 5,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "232b1f88-3fdd-4577-ac70-a75edaf8c32c",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-G",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "1",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "4.5000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-0-6": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "520",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 213.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "5.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 644036,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 6,
"SlicePosition": 10,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "565/24(G)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 0,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 320,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-G_006.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "320.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:26 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 6,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "3c3de3de-a1bc-48de-b2d9-05cb2affe9b7",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-G",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "1",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "4.5000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-0-7": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "520",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 223.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "5.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 644594,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 7,
"SlicePosition": 20,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "565/24(G)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 0,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 320,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-G_007.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "320.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:27 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 7,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "8db68c18-3c22-4f49-9c84-6876a775b60b",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-G",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "1",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "4.5000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-0-8": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "520",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 233.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "5.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 645201,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 8,
"SlicePosition": 30,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "565/24(G)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 0,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 320,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-G_008.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "320.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:27 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 8,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "43c8b643-e851-45f9-ac90-182f245956af",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-G",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "1",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "4.5000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-0-9": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "520",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 243.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "5.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 645771,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 9,
"SlicePosition": 40,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "565/24(G)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 0,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 320,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-G_009.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "320.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:28 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 9,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "07dcbb4d-1a88-4a78-82c8-752647148414",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-G",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "1",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "4.5000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-0-10": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "520",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 253.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "5.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 646344,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 10,
"SlicePosition": 50,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "565/24(G)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 0,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 320,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-G_010.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "320.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:29 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 10,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "915df4d4-e188-465b-ac5c-435f8ca42898",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-G",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "4.5000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-1-0": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "555",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 153.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 647690,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 0,
"SlicePosition": -50,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "585/11(T)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "4.5000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 1,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 160,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-T_000.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "160.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:30 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 0,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "5.0000",
"528-Name": "DA Shutter",
"555-State": "1",
"XLIGHT Dichroic-Label": "QUAD",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "547f0036-766b-4aa9-ad6a-7c5e739e7ba1",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-T",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-1-1": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "555",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 163.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 648147,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 1,
"SlicePosition": -40,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "585/11(T)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "4.5000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 1,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 160,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-T_001.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "160.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:30 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 1,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "5.0000",
"528-Name": "DA Shutter",
"555-State": "1",
"XLIGHT Dichroic-Label": "QUAD",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "1db8ea7d-6cb8-452d-aa23-c1ce8ef6c58c",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-T",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-1-2": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "555",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 173.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 648589,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 2,
"SlicePosition": -30,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "585/11(T)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "4.5000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 1,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 160,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-T_002.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "160.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:31 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 2,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "5.0000",
"528-Name": "DA Shutter",
"555-State": "1",
"XLIGHT Dichroic-Label": "QUAD",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "d941e8b9-21a5-43ad-8e4c-4db17183e195",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-T",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-1-3": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "555",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 183.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 649037,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 3,
"SlicePosition": -20,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "585/11(T)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "4.5000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 1,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 160,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-T_003.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "160.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:31 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 3,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "5.0000",
"528-Name": "DA Shutter",
"555-State": "1",
"XLIGHT Dichroic-Label": "QUAD",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "ed879477-b746-4741-8b32-304a2ea92a3c",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-T",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-1-4": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "555",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 193.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 649490,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 4,
"SlicePosition": -10,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "585/11(T)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "4.5000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 1,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 160,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-T_004.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "160.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:32 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 4,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "5.0000",
"528-Name": "DA Shutter",
"555-State": "1",
"XLIGHT Dichroic-Label": "QUAD",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "7d2156b8-3642-47da-8b43-10db8dd53b82",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-T",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-1-5": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "555",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 203.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 649884,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 5,
"SlicePosition": 0,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "585/11(T)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "4.5000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 1,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 160,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-T_005.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "160.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:32 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 5,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "5.0000",
"528-Name": "DA Shutter",
"555-State": "1",
"XLIGHT Dichroic-Label": "QUAD",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "23a5c8ff-16af-4291-bf71-d996dd33fcae",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-T",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-1-6": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "555",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 213.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 650285,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 6,
"SlicePosition": 10,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "585/11(T)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "4.5000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 1,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 160,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-T_006.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "160.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:32 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 6,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "5.0000",
"528-Name": "DA Shutter",
"555-State": "1",
"XLIGHT Dichroic-Label": "QUAD",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "0a0e5d04-1098-44f9-99f7-f87e31d89449",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-T",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-1-7": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "555",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 223.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 650685,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 7,
"SlicePosition": 20,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "585/11(T)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "4.5000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 1,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 160,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-T_007.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "160.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:33 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 7,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "5.0000",
"528-Name": "DA Shutter",
"555-State": "1",
"XLIGHT Dichroic-Label": "QUAD",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "8f897a84-42c3-449d-80e3-b3fd9cba2aa9",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-T",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-1-8": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "555",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 233.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 651090,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 8,
"SlicePosition": 30,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "585/11(T)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "4.5000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 1,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 160,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-T_008.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "160.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:33 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 8,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "5.0000",
"528-Name": "DA Shutter",
"555-State": "1",
"XLIGHT Dichroic-Label": "QUAD",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "61a08941-4c34-4b4e-8c06-d56d72da8dc3",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-T",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-1-9": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "555",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 243.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 651483,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 9,
"SlicePosition": 40,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "585/11(T)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "4.5000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 1,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 160,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-T_009.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "160.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:34 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 9,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "5.0000",
"528-Name": "DA Shutter",
"555-State": "1",
"XLIGHT Dichroic-Label": "QUAD",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "222a68de-5c0b-4e06-92fb-e117429dc757",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-T",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-1-10": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "555",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 253.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 651891,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 10,
"SlicePosition": 50,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "585/11(T)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "4.5000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 1,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 160,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-T_010.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "160.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:34 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 10,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "5.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "QUAD",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "6a6d52f3-abf2-440a-837a-fb27ccc69a2b",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-T",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-2-0": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "640",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 153.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 653009,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "1",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 0,
"SlicePosition": -50,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "679/29 (A)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "5.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 2,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 320,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-A_000.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "320.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "4.5000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:35 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 0,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "2ded63eb-b182-4af0-88c4-fef1b3d974cf",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-A",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-2-1": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "640",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 163.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 653580,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "1",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 1,
"SlicePosition": -40,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "679/29 (A)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "5.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 2,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 320,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-A_001.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "320.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "4.5000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:36 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 1,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "89ba5ed2-3628-46d2-a5b6-7a732da2c6b7",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-A",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-2-2": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "640",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 173.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 654184,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "1",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 2,
"SlicePosition": -30,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "679/29 (A)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "5.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 2,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 320,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-A_002.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "320.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "4.5000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:36 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 2,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "3ebc19a6-6244-495f-b3d9-85a5fd988bbb",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-A",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-2-3": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "640",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 183.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 654746,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "1",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 3,
"SlicePosition": -20,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "679/29 (A)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "5.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 2,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 320,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-A_003.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "320.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "4.5000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:37 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 3,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "542964a9-70a5-46c7-be59-ebb1f3085160",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-A",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-2-4": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "640",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 193.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 655356,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "1",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 4,
"SlicePosition": -10,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "679/29 (A)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "5.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 2,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 320,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-A_004.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "320.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "4.5000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:38 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 4,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "84893550-a987-4118-a73f-d4a8a455ef61",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-A",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-2-5": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "640",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 203.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 655918,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "1",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 5,
"SlicePosition": 0,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "679/29 (A)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "5.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 2,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 320,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-A_005.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "320.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "4.5000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:38 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 5,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "28ceda8c-5226-4026-a3d8-0443d9f7a002",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-A",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-2-6": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "640",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 213.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 656488,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "1",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 6,
"SlicePosition": 10,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "679/29 (A)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "5.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 2,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 320,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-A_006.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "320.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "4.5000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:39 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 6,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "bdd28964-cd3d-48ee-bf4f-da5dda785b9a",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-A",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-2-7": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "640",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 223.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 657050,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "1",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 7,
"SlicePosition": 20,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "679/29 (A)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "5.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 2,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 320,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-A_007.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "320.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "4.5000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:39 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 7,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "fe3f8c77-6ec0-48b9-a63e-bf0b4695ec34",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-A",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-2-8": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "640",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 233.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 657622,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "1",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 8,
"SlicePosition": 30,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "679/29 (A)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "5.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 2,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 320,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-A_008.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "320.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "4.5000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:40 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 8,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "0c6a4847-953a-410d-8720-6cf4abf5a375",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-A",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-2-9": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "640",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 243.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 658181,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "1",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 9,
"SlicePosition": 40,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "679/29 (A)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "5.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 2,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 320,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-A_009.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "320.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "4.5000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:40 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 9,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "ff98cf9b-5da7-4945-aa93-659a2d93945e",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-A",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-2-10": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "640",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 253.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 658794,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "1",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 10,
"SlicePosition": 50,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "679/29 (A)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "5.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 2,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 320,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-A_010.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "320.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "4.5000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:41 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 10,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "67db2ab7-655a-4705-9144-f3a51551a741",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-A",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-3-0": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "640",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 153.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 659920,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "1",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 0,
"SlicePosition": -50,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "725/40 (C)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "5.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 3,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 520,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-C_000.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "520.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "4.5000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:42 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 0,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "7060ab57-d5d0-4d1e-ba1c-30fa20ac4e7a",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-C",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-3-1": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "640",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 163.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 660683,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "1",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 1,
"SlicePosition": -40,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "725/40 (C)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "5.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 3,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 520,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-C_001.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "520.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "4.5000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:43 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 1,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "608615ea-e796-44e7-9847-be81056be6c8",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-C",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-3-2": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "640",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 173.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 661451,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "1",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 2,
"SlicePosition": -30,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "725/40 (C)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "5.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 3,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 520,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-C_002.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "520.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "4.5000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:44 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 2,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "b6dea229-68cf-4749-9b4d-8de9eff89c30",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-C",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-3-3": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "640",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 183.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 662203,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "1",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 3,
"SlicePosition": -20,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "725/40 (C)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "5.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 3,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 520,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-C_003.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "520.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "4.5000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:44 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 3,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "c0c21342-9898-48ab-a242-ca856ecf7b06",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-C",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-3-4": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "640",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 193.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 662954,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "1",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 4,
"SlicePosition": -10,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "725/40 (C)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "5.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 3,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 520,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-C_004.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "520.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "4.5000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:45 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 4,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "591d3888-b39a-4d40-b5bf-3edecb60164e",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-C",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-3-5": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "640",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 203.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 663712,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "1",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 5,
"SlicePosition": 0,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "725/40 (C)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "5.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 3,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 520,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-C_005.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "520.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "4.5000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:46 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 5,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "dfdfe337-6e34-45c0-9ece-c712a834e59d",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-C",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-3-6": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "640",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 213.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 664482,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "1",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 6,
"SlicePosition": 10,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "725/40 (C)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "5.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 3,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 520,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-C_006.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "520.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "4.5000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:47 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 6,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "4fbd0403-cf3d-46d0-b09f-aa102728eb87",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-C",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-3-7": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "640",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 223.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 665240,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "1",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 7,
"SlicePosition": 20,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "725/40 (C)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "5.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 3,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 520,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-C_007.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "520.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "4.5000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:47 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 7,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "0fa28331-4e15-44e6-bcce-0530222b7690",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-C",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-3-8": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "640",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 233.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 665990,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "1",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 8,
"SlicePosition": 30,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "725/40 (C)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "5.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 3,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 520,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-C_008.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "520.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "4.5000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:48 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 8,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "1de1ce6a-0dfa-45bf-93a6-c59afb494656",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-C",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-3-9": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "640",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 243.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 666749,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "1",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 9,
"SlicePosition": 40,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "725/40 (C)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "5.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 3,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 520,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-C_009.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "520.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "4.5000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:49 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 9,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "c1112e53-3176-4a5d-8ca7-5a50c6aae199",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-C",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-3-10": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "640",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 253.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 667560,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 10,
"SlicePosition": 50,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "725/40 (C)",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "5.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 3,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 520,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_Il-C_010.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "520.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "4.5000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:50 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 10,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "75b75b7a-d47b-4d2c-9cf4-cfcb7af3e21e",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "Il-C",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-4-0": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "Vincent-D3",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 153.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 668512,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "Excitation-2",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 0,
"SlicePosition": -50,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "Empty",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 4,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 20,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_P-DIC_000.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "20.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:51 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 0,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "de11585d-2efa-4440-a6ab-8800833cc826",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "P-DIC",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-4-1": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "Vincent-D3",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 163.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 668789,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "Excitation-2",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 1,
"SlicePosition": -40,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "Empty",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 4,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 20,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_P-DIC_001.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "20.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:51 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 1,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "26b884a1-25bc-4c3d-882d-a479ef465200",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "P-DIC",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-4-2": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "Vincent-D3",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 173.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 669056,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "Excitation-2",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 2,
"SlicePosition": -30,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "Empty",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 4,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 20,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_P-DIC_002.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "20.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:51 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 2,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "90e08d56-61a3-4790-889c-c28cb2eec55f",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "P-DIC",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-4-3": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "Vincent-D3",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 183.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 669379,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "Excitation-2",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 3,
"SlicePosition": -20,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "Empty",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 4,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 20,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_P-DIC_003.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "20.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:52 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 3,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "b18f6e87-09e7-475a-9a2c-91b1b4eee9f7",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "P-DIC",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-4-4": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "Vincent-D3",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 193.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 669651,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "Excitation-2",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 4,
"SlicePosition": -10,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "Empty",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 4,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 20,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_P-DIC_004.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "20.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:52 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 4,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "24ca89e6-2a02-44b8-8915-85df596d74b1",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "P-DIC",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-4-5": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "Vincent-D3",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 203.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 669909,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "Excitation-2",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 5,
"SlicePosition": 0,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "Empty",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 4,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 20,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_P-DIC_005.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "20.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:52 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 5,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "df25c5f6-aa74-4420-9add-56efe9e669f6",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "P-DIC",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-4-6": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "Vincent-D3",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 213.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 670174,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "Excitation-2",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 6,
"SlicePosition": 10,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "Empty",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 4,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 20,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_P-DIC_006.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "20.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:52 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 6,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "abadc94b-5b23-4f01-8987-dc64dd539dd6",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "P-DIC",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-4-7": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "Vincent-D3",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 223.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 670431,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "Excitation-2",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 7,
"SlicePosition": 20,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "Empty",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 4,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 20,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_P-DIC_007.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "20.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:53 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 7,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "291d04d1-8e04-4e8f-85a7-7ec7bcebb6c9",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "P-DIC",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-4-8": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "Vincent-D3",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 233.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 670686,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "Excitation-2",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 8,
"SlicePosition": 30,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "Empty",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 4,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 20,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_P-DIC_008.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "20.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:53 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 8,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "94b49b4e-d9b3-4e52-b806-b606d89a9f6d",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "P-DIC",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-4-9": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "Vincent-D3",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 243.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 670942,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "Excitation-2",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 9,
"SlicePosition": 40,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "Empty",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 4,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 20,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_P-DIC_009.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "20.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:53 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 9,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "d31cd8a3-2d60-4fd1-8d9f-ba0c44afe210",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "P-DIC",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-4-10": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "Vincent-D3",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 253.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "0.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "0.0000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 671264,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "Excitation-2",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 10,
"SlicePosition": 50,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "Empty",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 4,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 20,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_P-DIC_010.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "20.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:53 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 10,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "Di03-R635-t1",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "3401aad1-302e-4cd2-8373-d4912d27e03a",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "P-DIC",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-5-0": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "405",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 153.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "5.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "4.5000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 672602,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 0,
"SlicePosition": -50,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "QUAD",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 5,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 300,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_P-DAPI-high_000.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "300.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:55 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 0,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "QUAD",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "1",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "881bf656-58e8-420a-8a95-c20579f34618",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "P-DAPI-high",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-5-1": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "405",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 163.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "5.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "4.5000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 673142,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 1,
"SlicePosition": -40,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "QUAD",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 5,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 300,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_P-DAPI-high_001.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "300.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:55 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 1,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "QUAD",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "1",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "0d79cc32-16d9-45f5-bf8a-2dd06ef48a5b",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "P-DAPI-high",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-5-2": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "405",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 173.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "5.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "4.5000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 673683,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 2,
"SlicePosition": -30,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "QUAD",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 5,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 300,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_P-DAPI-high_002.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "300.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:56 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 2,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "QUAD",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "1",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "fce85d3b-6229-47b8-aaca-73e10ce40089",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "P-DAPI-high",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-5-3": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "405",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 183.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "5.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "4.5000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 674227,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 3,
"SlicePosition": -20,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "QUAD",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 5,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 300,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_P-DAPI-high_003.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "300.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:56 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 3,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "QUAD",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "1",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "42f48b2f-9e48-4c67-8e57-d0e15ffc3626",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "P-DAPI-high",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-5-4": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "405",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 193.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "5.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "4.5000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 674771,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 4,
"SlicePosition": -10,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "QUAD",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 5,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 300,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_P-DAPI-high_004.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "300.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:57 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 4,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "QUAD",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "1",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "f2a28ca4-b3c8-446c-bb04-129cc44c97e1",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "P-DAPI-high",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-5-5": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "405",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 203.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "5.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "4.5000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 675313,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 5,
"SlicePosition": 0,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "QUAD",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 5,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 300,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_P-DAPI-high_005.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "300.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:57 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 5,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "QUAD",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "1",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "a4055b52-136f-4956-82f7-31684899d828",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "P-DAPI-high",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-5-6": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "405",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 213.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "5.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "4.5000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 675856,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 6,
"SlicePosition": 10,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "QUAD",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 5,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 300,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_P-DAPI-high_006.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "300.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:58 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 6,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "QUAD",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "1",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "ea8749c7-ef2e-423c-906f-e96965a70049",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "P-DAPI-high",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-5-7": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "405",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 223.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "5.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "4.5000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 676401,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 7,
"SlicePosition": 20,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "QUAD",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 5,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 300,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_P-DAPI-high_007.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "300.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:59 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 7,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "QUAD",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "1",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "514246dd-dfe6-4228-8cf4-e828d219757e",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "P-DAPI-high",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-5-8": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "405",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 233.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "5.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "4.5000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 676948,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 8,
"SlicePosition": 30,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "QUAD",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 5,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 300,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_P-DAPI-high_008.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "300.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:15:59 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 8,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "QUAD",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "1",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "cf416b84-daef-4c9d-aa3c-e74e9cdfee43",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "P-DAPI-high",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-5-9": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "405",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 243.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "5.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "4.5000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 677497,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 9,
"SlicePosition": 40,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "QUAD",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 5,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 300,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_P-DAPI-high_009.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "300.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:16:00 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 9,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "QUAD",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "1",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "a9bcf57e-25b7-425f-94e7-c8f10044c8ae",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "P-DAPI-high",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
},
"FrameKey-0-5-10": {
"COM4-StopBits": "1",
"528 AO-MaxVolts": "5.0000",
"XLIGHT Excitation Wheel-State": "1",
"470 Shutter-Name": "AnalogIO",
"Core-Shutter": "405",
"555 AO-Demo": "No",
"XLIGHT Dichroic-State": "2",
"XYStage-StepSize-Y": "0.1000",
"ZPositionUm": 253.21,
"XYStage-StepSize-X": "0.1000",
"PRIME-FirmwareVersion": "20.28",
"PRIME-ClearCycles": "2",
"520-Name": "DA Shutter",
"405 Shutter-Name": "AnalogIO",
"PIEZO-StepSize": "0.0050",
"PRIME-ClearMode": "Pre-Exposure",
"470 AO-MinVolts": "0.0000",
"405 AO-SupportsTriggering": "1",
"CameraChannelIndex": 0,
"XYStage-StartSpeed": "500.0000",
"COM9-StopBits": "2",
"405 AO-Description": "NI DAC",
"COM4-DelayBetweenCharsMs": "0.0000",
"445 AO-Block voltage": "false",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"555 AO-Description": "NI DAC",
"520 AO-TriggerSampleRate": "100000",
"COM4-Verbose": "1",
"Vincent-D3-Description": "Vincent D3 controller adapter",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM4-Name": "COM4",
"405 Shutter-Volts": "5.0000",
"Vincent-D3-Port": "COM1",
"PRIME-PP 4 MIN ADU AFFECTED": "200",
"470 Shutter-MinVolts": "0.0000",
"PRIME-PP 3 ENABLED": "Yes",
"470 AO-Description": "NI DAC",
"PRIME-Description": "PVCAM API device adapter",
"520 Shutter-MaxVolts": "5.0000",
"520 Shutter-Volts": "0.0000",
"528 AO-Sequenceable": "1",
"PRIME-Binning": "1x1",
"555 Shutter-MaxVolts": "5.0000",
"528 Shutter-TriggerSequenceLength": "1000",
"405 Shutter-Block voltage": "false",
"XLIGHT Spin motor-State": "1",
"520 AO-AutoDetectedOutputPort": "Dev1/ao4",
"Vincent-D3-Time to open (ms)": "35.0000",
"Vincent-D3-Time to close (ms)": "35.0000",
"445 AO-TriggerSampleRate": "100000",
"640 Shutter-IOChannel": "Dev1/ao14",
"555 AO-Sequenceable": "1",
"520 AO-Name": "AnalogIO",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"470 AO-Block voltage": "false",
"COM1-Verbose": "1",
"COM9-AnswerTimeout": "10000.0000",
"528 Shutter-Name": "AnalogIO",
"PRIME-Trigger Timeout (secs)": "10",
"LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ",
"NextFrame": 0,
"470 AO-TriggerSequenceLength": "1000",
"PRIME-TransposeXY": "0",
"445 AO-TriggerSequenceLength": "1000",
"COM1-Name": "COM1",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"PRIME-PP 4 QUANTVIEW": "QUANTVIEW",
"640 AO-AutoDetectedOutputPort": "Dev1/ao7",
"COM9-DataBits": "8",
"COM1-AnswerTimeout": "500.0000",
"Camera": "",
"TE2000-Version": "V2.50-V2.50-V2.10",
"528 Shutter-Volts": "0.0000",
"XLIGHT Hub-Name": "XLIGHT Hub",
"PIEZO-Description": "Ludl stage driver adapter",
"PositionName": "Pos15",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSampleRate": "100000",
"PRIME-FanSpeedSetpoint": "High",
"FilterBlock-Description": "Nikon TE2000 filter block changeover adapter",
"405 AO-TriggerSampleRate": "100000",
"470 Shutter-Block voltage": "false",
"470-State": "0",
"COM3-Parity": "None",
"405 AO-Volts": "4.5000",
"PRIME-TransposeMirrorY": "0",
"PRIME-ChipName": "GS144BSI",
"470 AO-Volts": "0.0000",
"PRIME-TransposeMirrorX": "0",
"445 Shutter-SupportsTriggering": "0",
"470 Shutter-SupportsTriggering": "0",
"XLIGHT Hub-Description": "XLIGHT hub",
"PRIME-PreampOffLimit": "10000",
"470 AO-Sequenceable": "1",
"528 Shutter-MaxVolts": "5.0000",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao9",
"FilterBlock-Name": "FilterBlock",
"PRIME-CircularBufferFrameRecovery": "OFF",
"Core-XYStage": "XYStage",
"470 AO-TriggerSampleRate": "100000",
"PRIME-Timing-PreTriggerDelayNs": "24672000.0000",
"528-DA Device": "528 Shutter",
"Focus-Description": "Nikon TE2000 focus adapter",
"FilterBlock-State": "5",
"520 AO-Demo": "No",
"COM3-DelayBetweenCharsMs": "0.0000",
"470 Shutter-Volts": "0.0000",
"528 AO-AutoDetectedOutputPort": "Dev1/ao5",
"PRIME-PP 2 THRESHOLD": "125",
"PRIME-PP 4 THRESHOLD": "75",
"XYStage-Acceleration": "4.0000",
"470 Shutter-Demo": "No",
"PRIME-Name": "PMPCIECam00",
"640-DA Device": "640 Shutter",
"405 AO-Block voltage": "false",
"ElapsedTime-ms": 678082,
"PRIME-Offset": "100",
"445 AO-IOChannel": "Dev1/ao1",
"640 AO-MaxVolts": "5.0000",
"PositionIndex": 15,
"PRIME-PP 1 ENABLED": "Yes",
"COM1-BaudRate": "9600",
"640-State": "0",
"COM3-Verbose": "1",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"LudlController-TransmissionDelay": "0",
"PRIME-FullWellCapacity": "62000",
"PRIME-SMARTStreamingEnabled": "No",
"555 AO-AutoDetectedOutputPort": "Dev1/ao6",
"640 Shutter-Description": "NI DAC",
"528-Description": "DA device that is used as a shutter",
"COM4-Description": "Serial port driver (boost:asio)",
"405 Shutter-Description": "NI DAC",
"TE2000-Name": "TE2000",
"528 Shutter-Description": "NI DAC",
"COM9-Description": "Serial port driver (boost:asio)",
"LudlController-Name": "LudlController",
"445 Shutter-TriggerSequenceLength": "1000",
"PRIME-SerialNumber": "A18B203008",
"445 AO-Volts": "0.0000",
"PRIME-X-dimension": "1200",
"PRIME-ShutterCloseDelay": "0",
"XLIGHT Disk slider-State": "2",
"Position": "Default",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 AO-IOChannel": "Dev1/ao5",
"520 AO-IOChannel": "Dev1/ao4",
"528 AO-TriggerSequenceLength": "1000",
"405 Shutter-MaxVolts": "5.0000",
"470-DA Device": "470 Shutter",
"PRIME-FTCapable": "No",
"Core-ChannelGroup": "Channels",
"555 AO-SupportsTriggering": "0",
"PRIME-Y-dimension": "1200",
"Frame": 0,
"555 Shutter-Sequenceable": "1",
"PRIME-Timing-ExposureTimeNs": "499998000.0000",
"Core-ImageProcessor": "",
"528-State": "0",
"520 AO-Block voltage": "false",
"XLIGHT Excitation Wheel-Label": "QUAD",
"640 Shutter-Demo": "No",
"COM3-StopBits": "1",
"PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"520 Shutter-IOChannel": "Dev1/ao11",
"Width": 1200,
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-SupportsTriggering": "0",
"640 AO-TriggerSampleRate": "100000",
"520-Description": "DA device that is used as a shutter",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"405 Shutter-Sequenceable": "1",
"405 Shutter-SupportsTriggering": "0",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-TriggerSampleRate": "100000",
"640 Shutter-MaxVolts": "5.0000",
"555 AO-MaxVolts": "5.0000",
"520 AO-TriggerSequenceLength": "1000",
"COM3-BaudRate": "9600",
"COM9-BaudRate": "9600",
"Vincent-D3-Channel #": "Ch. #1",
"PRIME-PMode": "Normal",
"OpticalPath-Label": "Left(100)",
"BitDepth": 16,
"555 AO-TriggerSequenceLength": "1000",
"445 Shutter-Description": "NI DAC",
"405 AO-Sequenceable": "1",
"PRIME-AcquisitionMethod": "Callbacks",
"555 AO-Name": "AnalogIO",
"PRIME-ShutterMode": "Pre-Exposure",
"COM3-Handshaking": "Off",
"445 AO-SupportsTriggering": "0",
"ROI": "0-0-1200-1200",
"COM9-Verbose": "1",
"PRIME-Color": "OFF",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"PRIME-ShutterOpenDelay": "0",
"COM1-Parity": "None",
"Slice": 10,
"SlicePosition": 50,
"COM9-DelayBetweenCharsMs": "0.0000",
"555-Name": "DA Shutter",
"PIEZO-LudlSingleAxisName": "B",
"XLIGHT Emission Wheel-Label": "QUAD",
"640 Shutter-Name": "AnalogIO",
"640 AO-Sequenceable": "1",
"520 Shutter-Demo": "No",
"528 Shutter-Demo": "No",
"470-Name": "DA Shutter",
"520-DA Device": "520 Shutter",
"XYStage-Description": "Ludl XY stage driver adapter",
"445 Shutter-MinVolts": "0.0000",
"528 AO-TriggerSampleRate": "100000",
"405 AO-MinVolts": "0.0000",
"405 Shutter-TriggerSequenceLength": "1000",
"Height": 1200,
"555 AO-Block voltage": "false",
"640 Shutter-Volts": "0.0000",
"PixelType": "GRAY16",
"445 Shutter-Block voltage": "false",
"445 AO-MaxVolts": "5.0000",
"PRIME-PP 1 THRESHOLD": "125",
"520 Shutter-TriggerSequenceLength": "1000",
"COM9-Name": "COM9",
"Core-SLM": "",
"555 AO-Volts": "0.0000",
"555 Shutter-Block voltage": "false",
"XYStage-Speed": "1800.0000",
"520 AO-MaxVolts": "5.0000",
"528 Shutter-SupportsTriggering": "0",
"COM1-Description": "Serial port driver (boost:asio)",
"PRIME-MetadataEnabled": "No",
"520 Shutter-MinVolts": "0.0000",
"470 Shutter-Sequenceable": "1",
"PRIME-PreampDelay": "0",
"528 Shutter-IOChannel": "Dev1/ao12",
"555 Shutter-MinVolts": "0.0000",
"ChannelIndex": 5,
"PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"Core-AutoShutter": "1",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"Exposure-ms": 300,
"PRIME-PP 2 ENABLED": "Yes",
"640 Shutter-TriggerSampleRate": "100000",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"470 Shutter-IOChannel": "Dev1/ao10",
"405 AO-Demo": "No",
"PixelSizeUm": 1.1,
"470 AO-Demo": "No",
"470 Shutter-MaxVolts": "5.0000",
"Vincent-D3-Name": "Vincent-D3",
"PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"FileName": "img_000000000_P-DAPI-high_010.tif",
"LudlController-Reset": "Operate",
"PRIME-Exposure": "300.00",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"445-Name": "DA Shutter",
"555 Shutter-Name": "AnalogIO",
"520 Shutter-TriggerSampleRate": "100000",
"445 Shutter-IOChannel": "Dev1/ao9",
"PRIME-TransposeCorrection": "0",
"XYStage-StepSize": "0.1000",
"445-Description": "DA device that is used as a shutter",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 AO-IOChannel": "Dev1/ao7",
"520 Shutter-Name": "AnalogIO",
"COM3-DataBits": "8",
"640 AO-Volts": "0.0000",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"555 Shutter-IOChannel": "Dev1/ao13",
"WaitInterval": null,
"520 Shutter-Sequenceable": "1",
"YPositionUm": -13805.6,
"445-State": "0",
"445-DA Device": "445 Shutter",
"PRIME-CircularBufferAutoSize": "ON",
"OpticalPath-Description": "Nikon TE2000 optical path adapter",
"555 AO-TriggerSampleRate": "100000",
"445 Shutter-Volts": "0.0000",
"XLIGHT Hub-Port": "COM3",
"Core-AutoFocus": "",
"COM4-Handshaking": "Off",
"528 Shutter-MinVolts": "0.0000",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"445 Shutter-Name": "AnalogIO",
"528 Shutter-Sequenceable": "1",
"XYStage-TransposeMirrorX": "0",
"XYStage-TransposeMirrorY": "0",
"PRIME-ReadoutRate": "100MHz 16bit",
"XYStage-Name": "Stage",
"640 AO-SupportsTriggering": "0",
"640-Name": "DA Shutter",
"640 Shutter-Sequenceable": "1",
"PRIME-PP 2 MIN ADU AFFECTED": "200",
"640 AO-Block voltage": "false",
"445 Shutter-Sequenceable": "1",
"470 AO-MaxVolts": "5.0000",
"COM4-BaudRate": "9600",
"470-Description": "DA device that is used as a shutter",
"520 Shutter-Description": "NI DAC",
"PRIME-CCDTemperature": "-14.9200",
"Time": "2019-12-17 13:16:00 -0500",
"640 AO-TriggerSequenceLength": "1000",
"470 AO-Name": "AnalogIO",
"XLIGHT Disk slider-Label": "Disk Pos 40um",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Binning": "1x1",
"TE2000-ModelType": "1",
"COM3-AnswerTimeout": "500.0000",
"555 Shutter-SupportsTriggering": "0",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"TE2000-Port": "COM4",
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Description": "NI DAC",
"COM4-AnswerTimeout": "500.0000",
"COM1-DelayBetweenCharsMs": "0.0000",
"FilterBlock-Label": "Confocal",
"445 AO-Name": "AnalogIO",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"PRIME-Timing-ReadoutTimeNs": "24672000.0000",
"528 AO-Demo": "No",
"520 AO-Sequenceable": "1",
"555-Description": "DA device that is used as a shutter",
"405-DA Device": "405 Shutter",
"405 AO-TriggerSequenceLength": "1000",
"640 AO-MinVolts": "0.0000",
"640 Shutter-Block voltage": "false",
"FrameIndex": 0,
"PRIME-Port": "CMOS",
"555 AO-MinVolts": "0.0000",
"COM3-Description": "Serial port driver (boost:asio)",
"528 AO-Description": "NI DAC",
"555 AO-IOChannel": "Dev1/ao6",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"PRIME-ActualInterval-ms": "84.6200",
"LudlController-Port": "COM9",
"445 Shutter-Demo": "No",
"PRIME-Trigger-Expose Out-Mux": "1",
"SliceIndex": 10,
"PRIME-CCDTemperatureSetPoint": "-15.0000",
"405 Shutter-MinVolts": "0.0000",
"PRIME-ExposeOutMode": "First Row",
"AxisPositions": null,
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 Shutter-Volts": "0.0000",
"528-Name": "DA Shutter",
"555-State": "0",
"XLIGHT Dichroic-Label": "QUAD",
"445 AO-Sequenceable": "1",
"405-Name": "DA Shutter",
"405 AO-IOChannel": "Dev1/ao0",
"Core-Camera": "PRIME",
"Focus-Name": "Focus",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"405 Shutter-IOChannel": "Dev1/ao8",
"640 AO-Demo": "No",
"640 Shutter-SupportsTriggering": "0",
"445 AO-Demo": "No",
"555-DA Device": "555 Shutter",
"470 Shutter-Description": "NI DAC",
"528 AO-Volts": "0.0000",
"COM1-DataBits": "8",
"Vincent-D3-Address": "x",
"520 AO-SupportsTriggering": "0",
"405-Description": "DA device that is used as a shutter",
"640 Shutter-MinVolts": "0.0000",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"405 AO-Name": "AnalogIO",
"PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-TriggerSampleRate": "100000",
"470 Shutter-TriggerSequenceLength": "1000",
"COM1-StopBits": "1",
"520 Shutter-SupportsTriggering": "0",
"405-State": "0",
"Core-Initialize": "1",
"XLIGHT Emission Wheel-State": "3",
"520 AO-Description": "NI DAC",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"445 Shutter-MaxVolts": "5.0000",
"COM9-Handshaking": "Off",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XLIGHT Spin motor-Label": "On (spinning)",
"PRIME-PP 5 ENABLED": "No",
"UUID": "e2a627a9-5d5a-423b-845a-e5c0652e0cae",
"PRIME-TriggerMode": "Internal Trigger",
"COM1-Handshaking": "Off",
"PRIME-Timing-PostTriggerDelayNs": "49344000.0000",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"Focus-Position": "16732.9000",
"528 Shutter-Block voltage": "false",
"Channel": "P-DAPI-high",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"555 Shutter-Demo": "No",
"405 Shutter-Demo": "No",
"528 AO-Name": "AnalogIO",
"520-State": "0",
"Core-Galvo": "",
"405 Shutter-TriggerSampleRate": "100000",
"Focus-FocusStepSize_(nm)": "100",
"PRIME-PixelType": "16bit",
"COM4-DataBits": "8",
"470 AO-IOChannel": "Dev1/ao2",
"520 AO-MinVolts": "0.0000",
"COM9-Parity": "None",
"PRIME-CircularBufferEnabled": "ON",
"PRIME-PP 6 Reset": "No",
"640 AO-Description": "NI DAC",
"Source": "PRIME",
"445 AO-MinVolts": "0.0000",
"PIEZO-Name": "Stage",
"445 AO-Description": "NI DAC",
"PRIME-CircularBufferFrameCount": "46",
"PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"520 AO-Volts": "0.0000",
"528 Shutter-TriggerSampleRate": "100000",
"XPositionUm": 35352.6,
"OpticalPath-State": "4",
"PRIME-PP 3 THRESHOLD": "80",
"PRIME-PVCAM Version": "3.7.1",
"555 Shutter-TriggerSequenceLength": "1000",
"PRIME-Gain": "1-HDR",
"640 Shutter-TriggerSequenceLength": "1000",
"528 AO-SupportsTriggering": "0",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"OpticalPath-Name": "OpticalPath",
"PRIME-PP 4 ENABLED": "Yes",
"PRIME-PVCAM Adapter Version": "1.3.29",
"COM3-Name": "COM3",
"405 AO-MaxVolts": "5.0000",
"520 Shutter-Block voltage": "false",
"PRIME-Timing-ClearingTimeNs": "49344000.0000",
"Core-Focus": "PIEZO",
"528 AO-Block voltage": "false",
"TE2000-Description": "Nikon TE2000 hub",
"528 AO-MinVolts": "0.0000",
"COM4-Parity": "None",
"Summary": {
"Slices": 11,
"ChContrastMin": [
0,
0,
0,
0,
0,
0
],
"PixelAspect": 1,
"SlicesFirst": true,
"Interval_ms": 0,
"Time": "2019-12-17 13:15:23 -0500",
"Prefix": "seq_15",
"InitialPositionList": [
{
"Label": "Pos0",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [149.91],
"Focus": [16657.7],
"XYStage": [
71629.7,
19152.9
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos1",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [190.82],
"Focus": [16485.4],
"XYStage": [
65702.3,
19396.600000000002
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos2",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [194.69],
"Focus": [16446.8],
"XYStage": [
59880.5,
19007.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos3",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [145.04],
"Focus": [16446.6],
"XYStage": [
53616.9,
18818.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos4",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [169.57500000000002],
"Focus": [16446.5],
"XYStage": [
47662.8,
18817.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos5",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [131.805],
"Focus": [16446.4],
"XYStage": [
41918.8,
18298.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos6",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [113.145],
"Focus": [16446.5],
"XYStage": [
36129.9,
18895.8
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos7",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [141.005],
"Focus": [16446.6],
"XYStage": [
30299.100000000002,
18895.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos8",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [140.97],
"Focus": [16446.5],
"XYStage": [
23952.100000000002,
19156
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos9",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [184.57500000000002],
"Focus": [16677.9],
"XYStage": [
72866.8,
-14809.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos10",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [167.95000000000002],
"Focus": [16557.9],
"XYStage": [
66515.6,
-14371.300000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos11",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [168],
"Focus": [16618.9],
"XYStage": [
59724.3,
-13964.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos12",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.205],
"Focus": [16779.6],
"XYStage": [
53862.3,
-13494.7
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos13",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.20000000000002],
"Focus": [16749],
"XYStage": [
48108.3,
-13805.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos14",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.19],
"Focus": [16675.9],
"XYStage": [
41645,
-13804.900000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos15",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.21],
"Focus": [16675.7],
"XYStage": [
35352.6,
-13805.6
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos16",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.225],
"Focus": [16675.8],
"XYStage": [
29298,
-13447.400000000001
]
},
"GridColumnIndex": 0
},
{
"Label": "Pos17",
"GridRowIndex": 0,
"DeviceCoordinatesUm": {
"PIEZO": [203.215],
"Focus": [16675.8],
"XYStage": [
23269.100000000002,
-13012.300000000001
]
},
"GridColumnIndex": 0
}
],
"z-step_um": 10,
"GridRow": 0,
"BitDepth": 16,
"Depth": 2,
"Channels": 6,
"Source": "Micro-Manager",
"ChColors": [
-16711681,
-256,
-65281,
-1,
-1,
-16776961
],
"MetadataVersion": 10,
"KeepShutterOpenChannels": true,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536,
65536
],
"CustomIntervals_ms": [],
"Height": 1200,
"MicroManagerVersion": "1.4.23 20180219",
"GridColumn": 0,
"UUID": "6f58bae2-4ee1-4172-8c42-982c0c8b3a59",
"PixelType": "GRAY16",
"Width": 1200,
"Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq",
"Frames": 1,
"Comment": "",
"UserName": "Nikon",
"PixelSize_um": 1.1,
"ROI": [
0,
0,
1200,
1200
],
"Date": "2019-12-17",
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC",
"P-DAPI-high"
],
"KeepShutterOpenSlices": true,
"IJType": 1,
"ComputerName": "ZadorConfocal2",
"Positions": 18,
"PositionIndex": 15,
"CameraTimeout": 20000,
"TimeFirst": false
},
"640 AO-Name": "AnalogIO",
"Vincent-D3-Command": "Close"
}
}