{
 "cells": [
  {
   "cell_type": "code",
   "execution_count": 1,
   "id": "b1025a12",
   "metadata": {},
   "outputs": [],
   "source": [
    "import matplotlib.pyplot as plt\n",
    "import matplotlib.patches as patches\n",
    "from pathlib import Path\n",
    "import numpy as np\n",
    "import os\n",
    "import sys\n",
    "import glob\n",
    "import pandas as pd\n",
    "import xml.etree.ElementTree as et\n",
    "import datetime\n",
    "from imageio import volread as imread\n",
    "\n",
    "import tifffile\n",
    "\n",
    "# from pystackreg import StackReg --> don't run this, run this with imlab environment\n",
    "from skimage.filters import threshold_otsu\n",
    "\n",
    "import seaborn as sns\n",
    "#from pystackreg.util import to_uint16\n",
    "\n",
    "from skimage import measure\n",
    "from scipy import stats\n",
    "import umap\n",
    "import scanpy as sc\n",
    "#from umap.umap_ import UMAP"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 2,
   "id": "38700c6f",
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "/Volumes/imagestore/Brian/102022_D10_Coverslip2_Reimage_Processed\n"
     ]
    }
   ],
   "source": [
    "print(os.getcwd())"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 3,
   "id": "d65ef3c9",
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "194"
      ]
     },
     "execution_count": 3,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# Load Feature Data\n",
    "CP_DIR = 'cp_output'\n",
    "MP_DIR = 'mp_score_max'\n",
    "\n",
    "DATA_DIR = 'max_clean'\n",
    "Compartment = 'Soma'\n",
    "DATA_TYPE = 'max_clean'\n",
    "META_DIR = 'metadata'\n",
    "\n",
    "_allFOVs = sorted(glob.glob(f'{MP_DIR}/*'))\n",
    "allFOVs = [x.split('F')[-1][:3] for x in _allFOVs]\n",
    "len(allFOVs)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 4,
   "id": "ab32feb9",
   "metadata": {},
   "outputs": [
    {
     "ename": "FileNotFoundError",
     "evalue": "[Errno 2] No such file or directory: 'cp_output/AllFeat_SYTO_.csv'",
     "output_type": "error",
     "traceback": [
      "\u001b[0;31m---------------------------------------------------------------------------\u001b[0m",
      "\u001b[0;31mFileNotFoundError\u001b[0m                         Traceback (most recent call last)",
      "Input \u001b[0;32mIn [4]\u001b[0m, in \u001b[0;36m<cell line: 1>\u001b[0;34m()\u001b[0m\n\u001b[1;32m      1\u001b[0m \u001b[38;5;28;01mfor\u001b[39;00m compt \u001b[38;5;129;01min\u001b[39;00m [\u001b[38;5;124m'\u001b[39m\u001b[38;5;124mSYTO_\u001b[39m\u001b[38;5;124m'\u001b[39m,\u001b[38;5;124m'\u001b[39m\u001b[38;5;124mCytoplasm\u001b[39m\u001b[38;5;124m'\u001b[39m,\u001b[38;5;124m'\u001b[39m\u001b[38;5;124mFilteredNuclei\u001b[39m\u001b[38;5;124m'\u001b[39m]:\n\u001b[1;32m      2\u001b[0m     fname \u001b[38;5;241m=\u001b[39m \u001b[38;5;124mf\u001b[39m\u001b[38;5;124m'\u001b[39m\u001b[38;5;132;01m{\u001b[39;00mCP_DIR\u001b[38;5;132;01m}\u001b[39;00m\u001b[38;5;124m/AllFeat_\u001b[39m\u001b[38;5;132;01m{\u001b[39;00mcompt\u001b[38;5;132;01m}\u001b[39;00m\u001b[38;5;124m.csv\u001b[39m\u001b[38;5;124m'\u001b[39m\n\u001b[0;32m----> 3\u001b[0m     cp_df \u001b[38;5;241m=\u001b[39m \u001b[43mpd\u001b[49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[43mread_csv\u001b[49m\u001b[43m(\u001b[49m\u001b[43mfname\u001b[49m\u001b[43m)\u001b[49m\n\u001b[1;32m      4\u001b[0m     float64_cols \u001b[38;5;241m=\u001b[39m \u001b[38;5;28mlist\u001b[39m(cp_df\u001b[38;5;241m.\u001b[39mselect_dtypes(include\u001b[38;5;241m=\u001b[39m\u001b[38;5;124m'\u001b[39m\u001b[38;5;124mfloat64\u001b[39m\u001b[38;5;124m'\u001b[39m))\n\u001b[1;32m      5\u001b[0m     \u001b[38;5;28mprint\u001b[39m(sys\u001b[38;5;241m.\u001b[39mgetsizeof(cp_df))\n",
      "File \u001b[0;32m~/mambaforge/envs/imlab/lib/python3.9/site-packages/pandas/util/_decorators.py:311\u001b[0m, in \u001b[0;36mdeprecate_nonkeyword_arguments.<locals>.decorate.<locals>.wrapper\u001b[0;34m(*args, **kwargs)\u001b[0m\n\u001b[1;32m    305\u001b[0m \u001b[38;5;28;01mif\u001b[39;00m \u001b[38;5;28mlen\u001b[39m(args) \u001b[38;5;241m>\u001b[39m num_allow_args:\n\u001b[1;32m    306\u001b[0m     warnings\u001b[38;5;241m.\u001b[39mwarn(\n\u001b[1;32m    307\u001b[0m         msg\u001b[38;5;241m.\u001b[39mformat(arguments\u001b[38;5;241m=\u001b[39marguments),\n\u001b[1;32m    308\u001b[0m         \u001b[38;5;167;01mFutureWarning\u001b[39;00m,\n\u001b[1;32m    309\u001b[0m         stacklevel\u001b[38;5;241m=\u001b[39mstacklevel,\n\u001b[1;32m    310\u001b[0m     )\n\u001b[0;32m--> 311\u001b[0m \u001b[38;5;28;01mreturn\u001b[39;00m \u001b[43mfunc\u001b[49m\u001b[43m(\u001b[49m\u001b[38;5;241;43m*\u001b[39;49m\u001b[43margs\u001b[49m\u001b[43m,\u001b[49m\u001b[43m \u001b[49m\u001b[38;5;241;43m*\u001b[39;49m\u001b[38;5;241;43m*\u001b[39;49m\u001b[43mkwargs\u001b[49m\u001b[43m)\u001b[49m\n",
      "File \u001b[0;32m~/mambaforge/envs/imlab/lib/python3.9/site-packages/pandas/io/parsers/readers.py:680\u001b[0m, in \u001b[0;36mread_csv\u001b[0;34m(filepath_or_buffer, sep, delimiter, header, names, index_col, usecols, squeeze, prefix, mangle_dupe_cols, dtype, engine, converters, true_values, false_values, skipinitialspace, skiprows, skipfooter, nrows, na_values, keep_default_na, na_filter, verbose, skip_blank_lines, parse_dates, infer_datetime_format, keep_date_col, date_parser, dayfirst, cache_dates, iterator, chunksize, compression, thousands, decimal, lineterminator, quotechar, quoting, doublequote, escapechar, comment, encoding, encoding_errors, dialect, error_bad_lines, warn_bad_lines, on_bad_lines, delim_whitespace, low_memory, memory_map, float_precision, storage_options)\u001b[0m\n\u001b[1;32m    665\u001b[0m kwds_defaults \u001b[38;5;241m=\u001b[39m _refine_defaults_read(\n\u001b[1;32m    666\u001b[0m     dialect,\n\u001b[1;32m    667\u001b[0m     delimiter,\n\u001b[0;32m   (...)\u001b[0m\n\u001b[1;32m    676\u001b[0m     defaults\u001b[38;5;241m=\u001b[39m{\u001b[38;5;124m\"\u001b[39m\u001b[38;5;124mdelimiter\u001b[39m\u001b[38;5;124m\"\u001b[39m: \u001b[38;5;124m\"\u001b[39m\u001b[38;5;124m,\u001b[39m\u001b[38;5;124m\"\u001b[39m},\n\u001b[1;32m    677\u001b[0m )\n\u001b[1;32m    678\u001b[0m kwds\u001b[38;5;241m.\u001b[39mupdate(kwds_defaults)\n\u001b[0;32m--> 680\u001b[0m \u001b[38;5;28;01mreturn\u001b[39;00m \u001b[43m_read\u001b[49m\u001b[43m(\u001b[49m\u001b[43mfilepath_or_buffer\u001b[49m\u001b[43m,\u001b[49m\u001b[43m \u001b[49m\u001b[43mkwds\u001b[49m\u001b[43m)\u001b[49m\n",
      "File \u001b[0;32m~/mambaforge/envs/imlab/lib/python3.9/site-packages/pandas/io/parsers/readers.py:575\u001b[0m, in \u001b[0;36m_read\u001b[0;34m(filepath_or_buffer, kwds)\u001b[0m\n\u001b[1;32m    572\u001b[0m _validate_names(kwds\u001b[38;5;241m.\u001b[39mget(\u001b[38;5;124m\"\u001b[39m\u001b[38;5;124mnames\u001b[39m\u001b[38;5;124m\"\u001b[39m, \u001b[38;5;28;01mNone\u001b[39;00m))\n\u001b[1;32m    574\u001b[0m \u001b[38;5;66;03m# Create the parser.\u001b[39;00m\n\u001b[0;32m--> 575\u001b[0m parser \u001b[38;5;241m=\u001b[39m \u001b[43mTextFileReader\u001b[49m\u001b[43m(\u001b[49m\u001b[43mfilepath_or_buffer\u001b[49m\u001b[43m,\u001b[49m\u001b[43m \u001b[49m\u001b[38;5;241;43m*\u001b[39;49m\u001b[38;5;241;43m*\u001b[39;49m\u001b[43mkwds\u001b[49m\u001b[43m)\u001b[49m\n\u001b[1;32m    577\u001b[0m \u001b[38;5;28;01mif\u001b[39;00m chunksize \u001b[38;5;129;01mor\u001b[39;00m iterator:\n\u001b[1;32m    578\u001b[0m     \u001b[38;5;28;01mreturn\u001b[39;00m parser\n",
      "File \u001b[0;32m~/mambaforge/envs/imlab/lib/python3.9/site-packages/pandas/io/parsers/readers.py:933\u001b[0m, in \u001b[0;36mTextFileReader.__init__\u001b[0;34m(self, f, engine, **kwds)\u001b[0m\n\u001b[1;32m    930\u001b[0m     \u001b[38;5;28mself\u001b[39m\u001b[38;5;241m.\u001b[39moptions[\u001b[38;5;124m\"\u001b[39m\u001b[38;5;124mhas_index_names\u001b[39m\u001b[38;5;124m\"\u001b[39m] \u001b[38;5;241m=\u001b[39m kwds[\u001b[38;5;124m\"\u001b[39m\u001b[38;5;124mhas_index_names\u001b[39m\u001b[38;5;124m\"\u001b[39m]\n\u001b[1;32m    932\u001b[0m \u001b[38;5;28mself\u001b[39m\u001b[38;5;241m.\u001b[39mhandles: IOHandles \u001b[38;5;241m|\u001b[39m \u001b[38;5;28;01mNone\u001b[39;00m \u001b[38;5;241m=\u001b[39m \u001b[38;5;28;01mNone\u001b[39;00m\n\u001b[0;32m--> 933\u001b[0m \u001b[38;5;28mself\u001b[39m\u001b[38;5;241m.\u001b[39m_engine \u001b[38;5;241m=\u001b[39m \u001b[38;5;28;43mself\u001b[39;49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[43m_make_engine\u001b[49m\u001b[43m(\u001b[49m\u001b[43mf\u001b[49m\u001b[43m,\u001b[49m\u001b[43m \u001b[49m\u001b[38;5;28;43mself\u001b[39;49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[43mengine\u001b[49m\u001b[43m)\u001b[49m\n",
      "File \u001b[0;32m~/mambaforge/envs/imlab/lib/python3.9/site-packages/pandas/io/parsers/readers.py:1217\u001b[0m, in \u001b[0;36mTextFileReader._make_engine\u001b[0;34m(self, f, engine)\u001b[0m\n\u001b[1;32m   1213\u001b[0m     mode \u001b[38;5;241m=\u001b[39m \u001b[38;5;124m\"\u001b[39m\u001b[38;5;124mrb\u001b[39m\u001b[38;5;124m\"\u001b[39m\n\u001b[1;32m   1214\u001b[0m \u001b[38;5;66;03m# error: No overload variant of \"get_handle\" matches argument types\u001b[39;00m\n\u001b[1;32m   1215\u001b[0m \u001b[38;5;66;03m# \"Union[str, PathLike[str], ReadCsvBuffer[bytes], ReadCsvBuffer[str]]\"\u001b[39;00m\n\u001b[1;32m   1216\u001b[0m \u001b[38;5;66;03m# , \"str\", \"bool\", \"Any\", \"Any\", \"Any\", \"Any\", \"Any\"\u001b[39;00m\n\u001b[0;32m-> 1217\u001b[0m \u001b[38;5;28mself\u001b[39m\u001b[38;5;241m.\u001b[39mhandles \u001b[38;5;241m=\u001b[39m \u001b[43mget_handle\u001b[49m\u001b[43m(\u001b[49m\u001b[43m  \u001b[49m\u001b[38;5;66;43;03m# type: ignore[call-overload]\u001b[39;49;00m\n\u001b[1;32m   1218\u001b[0m \u001b[43m    \u001b[49m\u001b[43mf\u001b[49m\u001b[43m,\u001b[49m\n\u001b[1;32m   1219\u001b[0m \u001b[43m    \u001b[49m\u001b[43mmode\u001b[49m\u001b[43m,\u001b[49m\n\u001b[1;32m   1220\u001b[0m \u001b[43m    \u001b[49m\u001b[43mencoding\u001b[49m\u001b[38;5;241;43m=\u001b[39;49m\u001b[38;5;28;43mself\u001b[39;49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[43moptions\u001b[49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[43mget\u001b[49m\u001b[43m(\u001b[49m\u001b[38;5;124;43m\"\u001b[39;49m\u001b[38;5;124;43mencoding\u001b[39;49m\u001b[38;5;124;43m\"\u001b[39;49m\u001b[43m,\u001b[49m\u001b[43m \u001b[49m\u001b[38;5;28;43;01mNone\u001b[39;49;00m\u001b[43m)\u001b[49m\u001b[43m,\u001b[49m\n\u001b[1;32m   1221\u001b[0m \u001b[43m    \u001b[49m\u001b[43mcompression\u001b[49m\u001b[38;5;241;43m=\u001b[39;49m\u001b[38;5;28;43mself\u001b[39;49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[43moptions\u001b[49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[43mget\u001b[49m\u001b[43m(\u001b[49m\u001b[38;5;124;43m\"\u001b[39;49m\u001b[38;5;124;43mcompression\u001b[39;49m\u001b[38;5;124;43m\"\u001b[39;49m\u001b[43m,\u001b[49m\u001b[43m \u001b[49m\u001b[38;5;28;43;01mNone\u001b[39;49;00m\u001b[43m)\u001b[49m\u001b[43m,\u001b[49m\n\u001b[1;32m   1222\u001b[0m \u001b[43m    \u001b[49m\u001b[43mmemory_map\u001b[49m\u001b[38;5;241;43m=\u001b[39;49m\u001b[38;5;28;43mself\u001b[39;49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[43moptions\u001b[49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[43mget\u001b[49m\u001b[43m(\u001b[49m\u001b[38;5;124;43m\"\u001b[39;49m\u001b[38;5;124;43mmemory_map\u001b[39;49m\u001b[38;5;124;43m\"\u001b[39;49m\u001b[43m,\u001b[49m\u001b[43m \u001b[49m\u001b[38;5;28;43;01mFalse\u001b[39;49;00m\u001b[43m)\u001b[49m\u001b[43m,\u001b[49m\n\u001b[1;32m   1223\u001b[0m \u001b[43m    \u001b[49m\u001b[43mis_text\u001b[49m\u001b[38;5;241;43m=\u001b[39;49m\u001b[43mis_text\u001b[49m\u001b[43m,\u001b[49m\n\u001b[1;32m   1224\u001b[0m \u001b[43m    \u001b[49m\u001b[43merrors\u001b[49m\u001b[38;5;241;43m=\u001b[39;49m\u001b[38;5;28;43mself\u001b[39;49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[43moptions\u001b[49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[43mget\u001b[49m\u001b[43m(\u001b[49m\u001b[38;5;124;43m\"\u001b[39;49m\u001b[38;5;124;43mencoding_errors\u001b[39;49m\u001b[38;5;124;43m\"\u001b[39;49m\u001b[43m,\u001b[49m\u001b[43m \u001b[49m\u001b[38;5;124;43m\"\u001b[39;49m\u001b[38;5;124;43mstrict\u001b[39;49m\u001b[38;5;124;43m\"\u001b[39;49m\u001b[43m)\u001b[49m\u001b[43m,\u001b[49m\n\u001b[1;32m   1225\u001b[0m \u001b[43m    \u001b[49m\u001b[43mstorage_options\u001b[49m\u001b[38;5;241;43m=\u001b[39;49m\u001b[38;5;28;43mself\u001b[39;49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[43moptions\u001b[49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[43mget\u001b[49m\u001b[43m(\u001b[49m\u001b[38;5;124;43m\"\u001b[39;49m\u001b[38;5;124;43mstorage_options\u001b[39;49m\u001b[38;5;124;43m\"\u001b[39;49m\u001b[43m,\u001b[49m\u001b[43m \u001b[49m\u001b[38;5;28;43;01mNone\u001b[39;49;00m\u001b[43m)\u001b[49m\u001b[43m,\u001b[49m\n\u001b[1;32m   1226\u001b[0m \u001b[43m\u001b[49m\u001b[43m)\u001b[49m\n\u001b[1;32m   1227\u001b[0m \u001b[38;5;28;01massert\u001b[39;00m \u001b[38;5;28mself\u001b[39m\u001b[38;5;241m.\u001b[39mhandles \u001b[38;5;129;01mis\u001b[39;00m \u001b[38;5;129;01mnot\u001b[39;00m \u001b[38;5;28;01mNone\u001b[39;00m\n\u001b[1;32m   1228\u001b[0m f \u001b[38;5;241m=\u001b[39m \u001b[38;5;28mself\u001b[39m\u001b[38;5;241m.\u001b[39mhandles\u001b[38;5;241m.\u001b[39mhandle\n",
      "File \u001b[0;32m~/mambaforge/envs/imlab/lib/python3.9/site-packages/pandas/io/common.py:789\u001b[0m, in \u001b[0;36mget_handle\u001b[0;34m(path_or_buf, mode, encoding, compression, memory_map, is_text, errors, storage_options)\u001b[0m\n\u001b[1;32m    784\u001b[0m \u001b[38;5;28;01melif\u001b[39;00m \u001b[38;5;28misinstance\u001b[39m(handle, \u001b[38;5;28mstr\u001b[39m):\n\u001b[1;32m    785\u001b[0m     \u001b[38;5;66;03m# Check whether the filename is to be opened in binary mode.\u001b[39;00m\n\u001b[1;32m    786\u001b[0m     \u001b[38;5;66;03m# Binary mode does not support 'encoding' and 'newline'.\u001b[39;00m\n\u001b[1;32m    787\u001b[0m     \u001b[38;5;28;01mif\u001b[39;00m ioargs\u001b[38;5;241m.\u001b[39mencoding \u001b[38;5;129;01mand\u001b[39;00m \u001b[38;5;124m\"\u001b[39m\u001b[38;5;124mb\u001b[39m\u001b[38;5;124m\"\u001b[39m \u001b[38;5;129;01mnot\u001b[39;00m \u001b[38;5;129;01min\u001b[39;00m ioargs\u001b[38;5;241m.\u001b[39mmode:\n\u001b[1;32m    788\u001b[0m         \u001b[38;5;66;03m# Encoding\u001b[39;00m\n\u001b[0;32m--> 789\u001b[0m         handle \u001b[38;5;241m=\u001b[39m \u001b[38;5;28;43mopen\u001b[39;49m\u001b[43m(\u001b[49m\n\u001b[1;32m    790\u001b[0m \u001b[43m            \u001b[49m\u001b[43mhandle\u001b[49m\u001b[43m,\u001b[49m\n\u001b[1;32m    791\u001b[0m \u001b[43m            \u001b[49m\u001b[43mioargs\u001b[49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[43mmode\u001b[49m\u001b[43m,\u001b[49m\n\u001b[1;32m    792\u001b[0m \u001b[43m            \u001b[49m\u001b[43mencoding\u001b[49m\u001b[38;5;241;43m=\u001b[39;49m\u001b[43mioargs\u001b[49m\u001b[38;5;241;43m.\u001b[39;49m\u001b[43mencoding\u001b[49m\u001b[43m,\u001b[49m\n\u001b[1;32m    793\u001b[0m \u001b[43m            \u001b[49m\u001b[43merrors\u001b[49m\u001b[38;5;241;43m=\u001b[39;49m\u001b[43merrors\u001b[49m\u001b[43m,\u001b[49m\n\u001b[1;32m    794\u001b[0m \u001b[43m            \u001b[49m\u001b[43mnewline\u001b[49m\u001b[38;5;241;43m=\u001b[39;49m\u001b[38;5;124;43m\"\u001b[39;49m\u001b[38;5;124;43m\"\u001b[39;49m\u001b[43m,\u001b[49m\n\u001b[1;32m    795\u001b[0m \u001b[43m        \u001b[49m\u001b[43m)\u001b[49m\n\u001b[1;32m    796\u001b[0m     \u001b[38;5;28;01melse\u001b[39;00m:\n\u001b[1;32m    797\u001b[0m         \u001b[38;5;66;03m# Binary mode\u001b[39;00m\n\u001b[1;32m    798\u001b[0m         handle \u001b[38;5;241m=\u001b[39m \u001b[38;5;28mopen\u001b[39m(handle, ioargs\u001b[38;5;241m.\u001b[39mmode)\n",
      "\u001b[0;31mFileNotFoundError\u001b[0m: [Errno 2] No such file or directory: 'cp_output/AllFeat_SYTO_.csv'"
     ]
    }
   ],
   "source": [
    "for compt in ['SYTO_','Cytoplasm','FilteredNuclei']:\n",
    "    fname = f'{CP_DIR}/AllFeat_{compt}.csv'\n",
    "    cp_df = pd.read_csv(fname)\n",
    "    float64_cols = list(cp_df.select_dtypes(include='float64'))\n",
    "    print(sys.getsizeof(cp_df))\n",
    "    print(len(float64_cols), fname)\n",
    "    print('converting')\n",
    "    cp_df[float64_cols]=cp_df[float64_cols].astype('float32')\n",
    "    print(sys.getsizeof(cp_df))\n",
    "    cp_df.to_csv(f'{CP_DIR}/AllFeat_{compt}_32.csv', sep=',')"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "id": "4e5b614d",
   "metadata": {},
   "outputs": [],
   "source": []
  }
 ],
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    "name": "ipython",
    "version": 3
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   "file_extension": ".py",
   "mimetype": "text/x-python",
   "name": "python",
   "nbconvert_exporter": "python",
   "pygments_lexer": "ipython3",
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