{
 "cells": [
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": [
    "# tmat cycle 4\n"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 1,
   "metadata": {},
   "outputs": [],
   "source": [
    "import matplotlib.pyplot as plt\n",
    "import matplotlib.patches as patches\n",
    "from pathlib import Path\n",
    "import numpy as np\n",
    "import os\n",
    "import sys\n",
    "import glob\n",
    "import pandas as pd\n",
    "import xml.etree.ElementTree as et\n",
    "import datetime\n",
    "from skimage.io import imread, imsave\n",
    "from imageio import volread as imread\n",
    "\n",
    "import tifffile\n",
    "import pystackreg\n",
    "from pystackreg import StackReg\n",
    "from skimage.filters import threshold_otsu\n",
    "\n",
    "from pystackreg.util import to_uint16   # make sure version 0.2.5 (not anything below)\n",
    "from ims_to_tiff import convert_to_tif   "
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 4,
   "metadata": {},
   "outputs": [],
   "source": [
    "tmats = glob.glob('tmat_Cyc_4/*')"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 8,
   "metadata": {
    "scrolled": false
   },
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "tmat_Cyc_4/Cycle_4_F000_tmat.npy [[ 1.          0.         -3.4565662 ]\n",
      " [ 0.          1.          0.86930357]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F001_tmat.npy [[ 1.          0.         -3.45142272]\n",
      " [ 0.          1.          0.60330242]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F002_tmat.npy [[ 1.          0.         -3.45564015]\n",
      " [ 0.          1.          0.6469682 ]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F003_tmat.npy [[ 1.          0.         -3.42733281]\n",
      " [ 0.          1.          0.43746065]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F004_tmat.npy [[ 1.          0.         -3.40201384]\n",
      " [ 0.          1.          0.34173924]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F005_tmat.npy [[ 1.         0.        -3.4446607]\n",
      " [ 0.         1.         0.3279144]\n",
      " [ 0.         0.         1.       ]]\n",
      "tmat_Cyc_4/Cycle_4_F006_tmat.npy [[ 1.          0.         -3.49153177]\n",
      " [ 0.          1.          0.49236023]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F007_tmat.npy [[ 1.          0.         -3.45504459]\n",
      " [ 0.          1.          0.4133874 ]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F008_tmat.npy [[ 1.          0.         -3.49390658]\n",
      " [ 0.          1.          0.48953564]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F010_tmat.npy [[ 1.          0.         -4.04330083]\n",
      " [ 0.          1.          0.50028261]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F012_tmat.npy [[ 1.          0.         -4.05477871]\n",
      " [ 0.          1.          0.42606375]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F013_tmat.npy [[ 1.          0.         -4.07792177]\n",
      " [ 0.          1.          0.21938616]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F014_tmat.npy [[ 1.          0.         -4.04577109]\n",
      " [ 0.          1.          0.4331983 ]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F015_tmat.npy [[ 1.          0.         -3.42302621]\n",
      " [ 0.          1.          0.37548238]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F016_tmat.npy [[ 1.          0.         -3.39140004]\n",
      " [ 0.          1.          0.34300631]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F018_tmat.npy [[ 1.          0.         -3.37267624]\n",
      " [ 0.          1.          0.34246129]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F019_tmat.npy [[ 1.          0.         -3.43591149]\n",
      " [ 0.          1.          0.43171304]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F020_tmat.npy [[ 1.          0.         -3.44206954]\n",
      " [ 0.          1.          0.4438729 ]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F021_tmat.npy [[ 1.          0.         -3.44596276]\n",
      " [ 0.          1.          0.30566444]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F022_tmat.npy [[ 1.          0.         -3.43119923]\n",
      " [ 0.          1.          0.3803251 ]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F023_tmat.npy [[ 1.          0.         -3.42751074]\n",
      " [ 0.          1.          0.37027229]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F024_tmat.npy [[ 1.          0.         -3.39031169]\n",
      " [ 0.          1.          0.45210703]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F025_tmat.npy [[ 1.          0.         -3.39641424]\n",
      " [ 0.          1.          0.36466167]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F026_tmat.npy [[ 1.          0.         -3.40247088]\n",
      " [ 0.          1.         -0.75824529]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F027_tmat.npy [[ 1.          0.         -3.39552267]\n",
      " [ 0.          1.         -1.10547804]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F028_tmat.npy [[ 1.          0.         -3.43736819]\n",
      " [ 0.          1.          2.1128759 ]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F029_tmat.npy [[ 1.          0.         -3.37955193]\n",
      " [ 0.          1.         -1.54168877]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F030_tmat.npy [[ 1.          0.         -3.39758359]\n",
      " [ 0.          1.         -1.65127796]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F031_tmat.npy [[ 1.         0.        -3.3951079]\n",
      " [ 0.         1.         0.3733232]\n",
      " [ 0.         0.         1.       ]]\n",
      "tmat_Cyc_4/Cycle_4_F032_tmat.npy [[ 1.          0.         -3.41235263]\n",
      " [ 0.          1.          0.30176852]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F033_tmat.npy [[ 1.          0.         -3.45579283]\n",
      " [ 0.          1.          0.46154877]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F034_tmat.npy [[ 1.          0.         -3.46080672]\n",
      " [ 0.          1.          0.43787364]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F035_tmat.npy [[ 1.          0.         -3.2396479 ]\n",
      " [ 0.          1.          0.45162449]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F036_tmat.npy [[ 1.          0.         -3.25747412]\n",
      " [ 0.          1.         -0.67945086]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F037_tmat.npy [[ 1.          0.         -3.25404774]\n",
      " [ 0.          1.          0.2418424 ]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F038_tmat.npy [[ 1.          0.         -3.17064871]\n",
      " [ 0.          1.          0.19789068]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F040_tmat.npy [[ 1.          0.         -3.2557584 ]\n",
      " [ 0.          1.          0.28026407]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F041_tmat.npy [[ 1.          0.         -3.21349378]\n",
      " [ 0.          1.          0.36636692]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F042_tmat.npy [[ 1.          0.         -3.24685142]\n",
      " [ 0.          1.          0.238278  ]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F043_tmat.npy [[ 1.          0.         -3.22333583]\n",
      " [ 0.          1.          0.21928101]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F045_tmat.npy [[ 1.          0.         -3.21721962]\n",
      " [ 0.          1.          0.3828895 ]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F046_tmat.npy [[ 1.          0.         -3.20866234]\n",
      " [ 0.          1.         -0.73777855]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F047_tmat.npy [[ 1.          0.         -3.23403352]\n",
      " [ 0.          1.          0.28555937]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F048_tmat.npy [[ 1.          0.         -3.12558389]\n",
      " [ 0.          1.          0.32098288]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F049_tmat.npy [[ 1.          0.         -3.12474567]\n",
      " [ 0.          1.         -1.66501936]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F051_tmat.npy [[ 1.          0.         -3.2115992 ]\n",
      " [ 0.          1.          0.32584269]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F052_tmat.npy [[ 1.          0.         -3.14786359]\n",
      " [ 0.          1.          0.28693156]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F053_tmat.npy [[ 1.          0.         -3.1660106 ]\n",
      " [ 0.          1.         -0.01096218]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F054_tmat.npy [[ 1.          0.         -3.15511716]\n",
      " [ 0.          1.          0.17972867]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F055_tmat.npy [[ 1.          0.         -2.8374889 ]\n",
      " [ 0.          1.          0.14385288]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F056_tmat.npy [[ 1.          0.         -2.85021789]\n",
      " [ 0.          1.          0.30140762]\n",
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      "tmat_Cyc_4/Cycle_4_F057_tmat.npy [[ 1.          0.         -2.86218057]\n",
      " [ 0.          1.         -0.12058098]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F058_tmat.npy [[ 1.          0.         -2.88655789]\n",
      " [ 0.          1.          0.14865342]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F059_tmat.npy [[ 1.          0.         -2.93219898]\n",
      " [ 0.          1.          0.24484393]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F060_tmat.npy [[ 1.          0.         -3.67167478]\n",
      " [ 0.          1.          0.3867811 ]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F061_tmat.npy [[ 1.          0.         -3.6480694 ]\n",
      " [ 0.          1.          0.34202618]\n",
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      "tmat_Cyc_4/Cycle_4_F062_tmat.npy [[ 1.          0.         -3.65334866]\n",
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      "tmat_Cyc_4/Cycle_4_F063_tmat.npy [[ 1.          0.         -3.6518627 ]\n",
      " [ 0.          1.          0.04426729]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F064_tmat.npy [[ 1.          0.         -3.59536062]\n",
      " [ 0.          1.          0.23089345]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F065_tmat.npy [[ 1.          0.         -2.92719517]\n",
      " [ 0.          1.          0.1702176 ]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F066_tmat.npy [[ 1.          0.         -2.93884241]\n",
      " [ 0.          1.          0.3444256 ]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F067_tmat.npy [[ 1.          0.         -2.929351  ]\n",
      " [ 0.          1.          0.17917252]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F068_tmat.npy [[ 1.          0.         -2.92978407]\n",
      " [ 0.          1.          0.1586443 ]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F069_tmat.npy [[ 1.          0.         -2.92531709]\n",
      " [ 0.          1.          0.30656992]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F070_tmat.npy [[ 1.          0.         -3.01739181]\n",
      " [ 0.          1.          0.32619453]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F071_tmat.npy [[ 1.          0.         -3.02549702]\n",
      " [ 0.          1.          0.23944711]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F072_tmat.npy [[ 1.          0.         -2.98162173]\n",
      " [ 0.          1.          0.11850634]\n",
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      " [ 0.          1.         -0.0398557 ]\n",
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      "tmat_Cyc_4/Cycle_4_F074_tmat.npy [[ 1.          0.         -3.00128874]\n",
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      "tmat_Cyc_4/Cycle_4_F076_tmat.npy [[ 1.          0.         -2.90851349]\n",
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      " [ 0.          1.          0.14377048]\n",
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      "tmat_Cyc_4/Cycle_4_F081_tmat.npy [[ 1.          0.         -2.87494623]\n",
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      "tmat_Cyc_4/Cycle_4_F082_tmat.npy [[ 1.          0.         -2.85673117]\n",
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      "tmat_Cyc_4/Cycle_4_F087_tmat.npy [[ 1.          0.         -2.74596377]\n",
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      "tmat_Cyc_4/Cycle_4_F088_tmat.npy [[ 1.          0.         -2.72516513]\n",
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      "tmat_Cyc_4/Cycle_4_F089_tmat.npy [[ 1.          0.         -2.73071172]\n",
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      "tmat_Cyc_4/Cycle_4_F090_tmat.npy [[ 1.          0.         -3.38346323]\n",
      " [ 0.          1.          0.12767852]\n",
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      "tmat_Cyc_4/Cycle_4_F091_tmat.npy [[ 1.          0.         -3.39856318]\n",
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      "tmat_Cyc_4/Cycle_4_F092_tmat.npy [[ 1.          0.         -3.4120431 ]\n",
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      "tmat_Cyc_4/Cycle_4_F093_tmat.npy [[ 1.          0.         -3.4284508 ]\n",
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      "tmat_Cyc_4/Cycle_4_F094_tmat.npy [[ 1.          0.         -3.43734123]\n",
      " [ 0.          1.          0.22473248]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F095_tmat.npy [[ 1.          0.         -2.75232752]\n",
      " [ 0.          1.          0.28554357]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F096_tmat.npy [[ 1.00000000e+00  0.00000000e+00 -2.76755639e+00]\n",
      " [ 0.00000000e+00  1.00000000e+00 -4.15061482e-05]\n",
      " [ 0.00000000e+00  0.00000000e+00  1.00000000e+00]]\n",
      "tmat_Cyc_4/Cycle_4_F097_tmat.npy [[ 1.          0.         -2.7914423 ]\n",
      " [ 0.          1.          0.25326701]\n",
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      "tmat_Cyc_4/Cycle_4_F099_tmat.npy [[ 1.          0.         -2.74960305]\n",
      " [ 0.          1.          0.10000246]\n",
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      "tmat_Cyc_4/Cycle_4_F100_tmat.npy [[ 1.          0.         -1.70780986]\n",
      " [ 0.          1.          0.26467387]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F101_tmat.npy [[ 1.          0.         -1.77356214]\n",
      " [ 0.          1.          1.35858355]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F102_tmat.npy [[ 1.          0.         -1.83833957]\n",
      " [ 0.          1.          1.53722485]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F103_tmat.npy [[ 1.          0.         -1.71466096]\n",
      " [ 0.          1.          0.35086312]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F104_tmat.npy [[ 1.          0.         -1.71995367]\n",
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      "tmat_Cyc_4/Cycle_4_F210_tmat.npy [[ 1.          0.         -1.92034233]\n",
      " [ 0.          1.          0.21244238]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F211_tmat.npy [[ 1.          0.         -1.90247758]\n",
      " [ 0.          1.         -1.11949293]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F212_tmat.npy [[ 1.          0.         -1.90489459]\n",
      " [ 0.          1.          0.16350636]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F213_tmat.npy [[ 1.          0.         -1.87514022]\n",
      " [ 0.          1.          0.05261399]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F214_tmat.npy [[ 1.          0.         -1.86880563]\n",
      " [ 0.          1.         -0.03471226]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F216_tmat.npy [[ 1.          0.         -1.9653039 ]\n",
      " [ 0.          1.          0.24787049]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F217_tmat.npy [[ 1.          0.         -1.91928937]\n",
      " [ 0.          1.         -0.04866028]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F218_tmat.npy [[ 1.          0.         -2.01792329]\n",
      " [ 0.          1.          0.17343293]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F219_tmat.npy [[ 1.          0.         -1.95313315]\n",
      " [ 0.          1.          0.12166406]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F220_tmat.npy [[ 1.          0.         -1.75310799]\n",
      " [ 0.          1.          0.1629827 ]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F221_tmat.npy [[ 1.          0.         -1.81242748]\n",
      " [ 0.          1.         -0.22132348]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F222_tmat.npy [[ 1.          0.         -1.77937509]\n",
      " [ 0.          1.          0.0563247 ]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F223_tmat.npy [[ 1.          0.         -1.75041499]\n",
      " [ 0.          1.         -0.36312293]\n",
      " [ 0.          0.          1.        ]]\n",
      "tmat_Cyc_4/Cycle_4_F224_tmat.npy [[ 1.00000000e+00  0.00000000e+00 -1.82997483e+00]\n",
      " [ 0.00000000e+00  1.00000000e+00 -1.49099220e-03]\n",
      " [ 0.00000000e+00  0.00000000e+00  1.00000000e+00]]\n"
     ]
    }
   ],
   "source": [
    "for i in tmats:\n",
    "    x = np.load(i)\n",
    "    print(i, x)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 9,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "array([[  1.        ,   0.        , -98.97177198],\n",
       "       [  0.        ,   1.        , 166.55290812],\n",
       "       [  0.        ,   0.        ,   1.        ]])"
      ]
     },
     "execution_count": 9,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "np.load('tmat_Cyc_4/Cycle_4_F196_tmat.npy')"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": []
  }
 ],
 "metadata": {
  "kernelspec": {
   "display_name": "Python 3",
   "language": "python",
   "name": "python3"
  },
  "language_info": {
   "codemirror_mode": {
    "name": "ipython",
    "version": 3
   },
   "file_extension": ".py",
   "mimetype": "text/x-python",
   "name": "python",
   "nbconvert_exporter": "python",
   "pygments_lexer": "ipython3",
   "version": "3.7.4"
  }
 },
 "nbformat": 4,
 "nbformat_minor": 2
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